动力相关蛋白Drp1在羊瘙痒因子139A感染小鼠脑组织中变化的研究  

Alteration of dynamin-related protein 1( Drp1 ) in brain tissue from scrapie-139A infected mice

作　　者：杨晓东[1] 陈利娜[1] 马月[1] 董小平[1] 石琦[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《疾病监测》2016年第5期374-379,共6页Disease Surveillance

基　　金：国家自然科学基金(No.81572048;81301429);传染病预防控制国家重点实验室基金(No.2015SKLID503)~~

摘　　要：目的研究动力相关蛋白1(Drp1)在羊瘙痒因子139A感染的小鼠脑组织中的变化。方法采用Western Blot方法检测Drp1在羊瘙痒因子139A感染的脑组织匀浆中的含量变化及其亚硝基化水平。采用组织免疫荧光方法检测Drp1在脑组织中的分布。结果在羊瘙痒因子139A感染终末期小鼠脑组织匀浆中,Drp1总量略有降低。对感染不同时间点的动态分析结果显示Drp1含量呈逐渐降低趋势,终末期稍有回升。感染终末期脑组织中亚硝基化水平明显增高。组织免疫荧光显示正常和羊瘙痒因子139A感染终末期小鼠脑组织中,Drp1与神经元细胞存在共定位现象。结论Drp1在小鼠中枢神经系统中主要分布于神经元细胞,在羊瘙痒因子139A感染的小鼠脑组织中,Drp1总量略有降低,另外亚硝基化水平明显增高,提示在羊瘙痒病感染的小鼠脑组织中,线粒体动力相关蛋白的表达量及翻译后修饰水平出现了变化,在感染过程中起着重要作用。Objective To investigate the alteration and its mechanism of dynamin-related protein 1（ Drp1） in the brain tissue from scrapie-139 A infected mice. Methods Western Blot assay was conducted to analyze the expression level and SNitrosylation level of Drp1. Immunofluorescent analyses was performed to study the distribution of Drp1 in brain tissue cells.Results The level of Drp1 in the brains of scrapie 139 A infected mice at terminal stage slightly decreased. Dynamic analysis of Drp1 levels in 139 A infected mice brains showed the same decline trend. The level of S-nitrosylated Drp1 increased remarkably in the brains of scrapie infected mice at terminal stage. Immunofluorescent analyses showed that Drp1 colocalized with the Neu N-positive cells in the brains of both normal and scrapie infected mice at terminal stage. Conclusion Drp1 was mainly distributed in neurons of the central nervous system,and in the brain tissues of either normal mice orscrapie-infected mice,Drp1 might adjust the fission of the mitochondria through the alteration of its distribution and / or protein modification after translation.

关 键 词：线粒体动力相关蛋白1 羊瘙痒因子 亚硝基化蛋白 

分 类 号：R742.9[医药卫生—神经病学与精神病学]