NAD^+抑制脂多糖诱导海马炎症因子的表达  

NAD^+ suppressed the expressions of inflammation factors in the hippocampus induced by lipopolysaccharide

作　　者：刘娟[1] 姚天佑 孔莺莺 胡伟伟[2] 王云[1] 李胜天[2] 

机构地区：[1]复旦大学脑科学研究院,上海200032 [2]上海交通大学生命科学技术学院,上海200240

出　　处：《扬州大学学报（农业与生命科学版）》2016年第1期16-21,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：国家自然科学基金资助项目(31271198);上海市自然科学基金重大项目(13DJ1400303)

摘　　要：采用1 mg·kg^(-1)脂多糖(LPS)腹腔注射30 min后进行烟酰胺腺嘌呤二核苷酸(NAD^+,腹腔注射100、300mg·kg-1)干预,并在LPS注射3和6h后,采用实时定量PCR方法检测海马中白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的mRNA表达量,探讨NAD^+干预对LPS诱导的C57BL/6雄性小鼠海马炎症因子IL-1β和TNF-αmRNA表达量变化的影响。结果表明:LPS诱导海马中IL-1β和TNF-αmRNA表达量明显升高;LPS注射3h后,NAD^+(100、300mg·kg^(-1))均显著抑制了LPS诱导的海马中IL-1βmRNA表达量的升高;LPS注射6h后,NAD+(100mg·kg^(-1))明显抑制了LPS诱导的海马中TNF-αmRNA表达量的升高。说明NAD+抑制LPS诱导的海马炎症因子的表达,提示NAD+可用于由脑部炎症引起的脑疾病早期干预治疗。Lipopolysaccharide（LPS,1mg·kg（-1））was injected intraperitoneally,and mice were treated withβ-Nicotinamide adenine dinucleotide（NAD＋,100,300mg·kg（-1））30min after the LPS injection.The changes in expressions of interlukin-1β（IL-1β）and tumor necrosis factor-α（TNF-α）mRNA in hippocampus at 3 and 6 hafter LPS injection,respectively,were measured by quantitative real-time PCR.The effect of NAD＋on the expressions of LPS-induced inflammation factors,IL-1 β and TNF-α,in the hippocampus of C57BL/6 mice was investigated.Results showed that：LPS led to the dramatic elevation of IL-1β and TNF-α mRNA in hippocampus.NAD＋（100,300 mg·kg（-1））markedly inhibited the augment of IL-1 β mRNA at 3 hafter LPS injection.NAD＋（100 mg·kg-1）significantly suppressed the elevation of TNF-αmRNA at 6 hafter LPS administration.The data demonstrated that NAD＋suppressed the expressions of inflammation factors in hippocampus induced by LPS,implying the application prospect of NAD＋in early-stage treatment of encephalitis or other brain diseases caused by cerebral inflammation factors.

关 键 词：β-烟酰胺腺嘌呤二核苷酸 脂多糖 白细胞介素-1Β 肿瘤坏死因子-Α 

分 类 号：R747[医药卫生—神经病学与精神病学]