Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole(Cynoglossus semilaevis)  

作　　者：DONG Zhongdian QI Qian ZHANG Ning SHAO Changwei ZHANG Liyan WEN Haishen CHEN Songlin 

机构地区：[1]Fisheries College,Ocean University of China [2]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences [3]Function Laboratory for Marine Fisheries Science and Food Production Processes,National Laboratory for Marine Science and Technology

出　　处：《Acta Oceanologica Sinica》2016年第6期19-28,共10页海洋学报（英文版）

基　　金：The National Natural Science Foundation under contract Nos 31130057 and 31472269;the Taishan Scholar Project Fund of Shandong of China

摘　　要：The signaling molecule hedgehog（Hh） is essential for cellular signaling required for body pattern formation in embryonic and individual development in vertebrate and invertebrates.Patched 1（Ptc1）,a receptor of Hh,mediates the activity of Hh by binging the Hh ligands.In this study,the Ptc1 of half-smooth tongue sole Cynoglossus semilaevis（CsPtc1） was cloned and characterized.The full-length of CsPtc1 cDNA is 5 212 nucleotides（nt）,and encodes a protein with 1 543 amino acid residues.CsPtc1 shared many features with Ptc1 of other telesot species.Real-time quantitative PCR revealed CsPtc1 mRNA expressed in brain,liver,heart,gill,intestines,spleen,gonad and kidney,with expression level in testis significantly higher than in ovary.In testis,hybridization signals were mainly detected in primary spermatocytes,secondary spermatocytes,and sertoli cells,while weak signals were found in oocytes.Correspondingly,the degree of methylation is higher in female than in male and pseudomale.Results indicate that CsPtc1 may be involved in Desert Hedgehog（DHH） maintenance of the male and pseudo-male germ line and spermatogenesis.The signaling molecule hedgehog（Hh） is essential for cellular signaling required for body pattern formation in embryonic and individual development in vertebrate and invertebrates.Patched 1（Ptc1）,a receptor of Hh,mediates the activity of Hh by binging the Hh ligands.In this study,the Ptc1 of half-smooth tongue sole Cynoglossus semilaevis（CsPtc1） was cloned and characterized.The full-length of CsPtc1 cDNA is 5 212 nucleotides（nt）,and encodes a protein with 1 543 amino acid residues.CsPtc1 shared many features with Ptc1 of other telesot species.Real-time quantitative PCR revealed CsPtc1 mRNA expressed in brain,liver,heart,gill,intestines,spleen,gonad and kidney,with expression level in testis significantly higher than in ovary.In testis,hybridization signals were mainly detected in primary spermatocytes,secondary spermatocytes,and sertoli cells,while weak signals were found in oocytes.Correspondingly,the degree of methylation is higher in female than in male and pseudomale.Results indicate that CsPtc1 may be involved in Desert Hedgehog（DHH） maintenance of the male and pseudo-male germ line and spermatogenesis.

关 键 词：half-smooth tongue sole male and female Patched 1 RACE QRT-PCR METHYLATION 

分 类 号：S917.4[农业科学—水产科学]