27株牛源肺炎克雷伯菌gyrA和parC基因遗传分析  

gyrA and parC genetic analysis of 12 strains of Klebsiella pneumonia from bovine

作　　者：刀丽梅 黄攀[1] 程方俊[1] 曹立亭[1] 林雅[1] 张婷婷[1] 林永润[1] 单文杰[1] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆402460

出　　处：《中国兽医学报》2016年第6期961-963,970,共4页Chinese Journal of Veterinary Science

基　　金：重庆市重点科技攻关资助项目(cstc2012jcsfjfzhX0021);西南大学博士启动基金资助项目(2013BSr03)

摘　　要：为了解重庆和四川地区牛源肺炎克雷伯菌分离株之间的进化关系,本试验通过PCR方法扩增27株肺炎克雷伯菌gyrA、parC基因并测序,对测序结果进行基因进化分析。结果表明,gyrA、parC基因片段扩增产物大小分别为420,382bp;27株分离株可分成4个大簇,荣昌分离株主要与ha06(中国·江苏)、NCTC11228(荷兰·乌特勒支)等聚类于Ⅰ簇;丰都分离株紧密聚类于Ⅱ簇;Ⅲ簇全为云阳分离株;自贡分离株聚类于Ⅰ、Ⅳ簇。The purpose of this study was to analyze the genetic evolution relationship of 12 strains of Klebsiella pneumonia,which isolated from bovine in Chongqing and Sichuan.PCR amplifications were applied to detect the gene sequences of gyrAand parCfor analysis of genetic evolution in all of these strains.The results indicated that the sizes of gyrA and parC gene amplification products were 420 bp and 382 bp,respectively.All the Klebsiella pneumonia strains could be divided into four large clusters：Rongchang isolates,ha06（China.Jiangsu）and NCTC11228（The Netherlands.Utrecht）clustered mainly in clusterⅠ;Fengdu isolates closely clustered in clusterⅡ;Yunyang isolates all belonged to clusterⅢ;Zigong isolates clustered in clusterⅠand Ⅳ.

关 键 词：牛源肺炎克雷伯菌 基因进化 GYRA基因 PARC基因 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]