沙枣中总黄酮的提取工艺优选  

Optimization of Extraction Process for Total Flavonoids from Elaeagnus angustifolia

作　　者：王红玉[1] 本莲芳 王安琪[1] 张海亮[1] 

机构地区：[1]兰州石化职业技术学院,甘肃兰州730060

出　　处：《广州化工》2016年第11期123-125,共3页GuangZhou Chemical Industry

摘　　要：优选沙枣中总黄酮的提取工艺条件,并通过还原力的测定初步研究黄酮的抗氧化活性。以总黄酮提取率为指标,采用单因素考察乙醇浓度、超声时间、料液比、超声功率对沙枣黄酮提取率的影响;在单因素试验的基础上,采用正交设计法优化沙枣中总黄酮的提取工艺条件。沙枣黄酮提取的最佳工艺条件:乙醇浓度60%、超声时间15 min、料液比1∶22(g/m L)、超声功率320 W,在此条件下,沙枣黄酮的提取率8.64%,影响沙枣黄酮提取率的因素从大到小依次为:料液比>超声时间>乙醇浓度>超声功率;通过还原力的测定,表明沙枣总黄酮有较好的抗氧化活性。To optimize extraction conditions of extracting total flavonoids from Elaeagnus angustifolia and preliminarily investigate antioxidant activity of flavonoids by measuring restoring force,with the extraction efficiency of total flavonoids as index,the effects the concentration of ethanol,ultrasonic time,solid-liquid ratio and ultrasonic power exerted on yield of total flavonoids extracted from Elaeagnus angustifolia were investigated by the single-factor test. Based on the single factor test,extraction technology of extracting total flavonoids from Elaeagnus angustifolia was optimized through orthogonal test. The optimum extraction technology conditions were as follows： alcohol concentration of 60%,ultrasonic time 15 min,solid-liquid ratio 1 ∶22（ g/m L）,ultrasound power 320 W. Under these conditions,the yield of total flavonoids reached to 8. 64%. The main factors that influenced the flavonoid extraction rate were listed in descending order as follows： solid-liquid ratio 〉 ultrasonic time 〉 alcohol concentration 〉 ultrasonic power. The restoring force measuring results showed that Elaeagnus angustifolia possessed excellent antioxidant activity.

关 键 词：沙枣 总黄酮 提取工艺 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]