辛苍颗粒中药材的定性鉴别和黄芩苷的含量测定  

Qualitative identification of Xincang Granules and determination of Baicalin in Xincang Granules

作　　者：陈华[1] 于波涛[1] 范开华[1] 金伟华[1] 蒲志强[1] 王诗华[1] 谢秋[1] 

机构地区：[1]解放军成都军区总医院药剂科,四川成都610083

出　　处：《西部医学》2016年第6期855-858,共4页Medical Journal of West China

基　　金：总后卫生部军队医疗机构制剂标准提高科研专项课题(14ZJZ18-2)

摘　　要：目的建立辛苍颗粒质量控制标准。方法采用薄层色谱法（TLC）对辛苍颗粒处方中的黄芩、甘草、黄芪进行定性鉴别;采用高效液相色谱法（HPLC）对处方中的黄芩苷进行含量测定,色谱柱为Agilent HC-C18（4.6mm×250mm,5μm）,流动相为甲醇-0.1%磷酸（40∶60）,流速为1.0ml/min,检测波长为280nm,柱温为34℃。结果 TLC斑点清晰,分离度较好,专属性强,阴性对照无干扰;黄芩苷在1.92-61.44μg/ml范围内浓度与峰面积呈良好的线性关系（r=0.9999）,平均回收率为100.30%,RSD=1.82%（n=9）。结论该研究方法准确可靠,重复性好,且简便可行,可用于辛苍颗粒的质量控制。Objective To establish the quality standard of Xincang Granules.Methods The TLC method was used to qualitatively identify Radix Scutellariae,licorice and Astragali Radix.HPLC method was adopted to determine the content article of baicalin.The determination was performed on Agilent HC-C18（4.6mm ×250mm,5μm）column with mobile phase consisted on methyl alcohol-0.1% phosphoric acid（40∶60）at the flow rate of 1.0ml/min,the column temparature was 34℃ and the detection wavelength was set at 280 nm.Results The spots of TLC were fairly clear with the good separation.The negative samples showed no interference.The linear range of baicalin was 1.92-61.44μg/ml（r=0.9999）.The average recovery rate was 100.30%.The RSD was 1.82%（n=9）.Conclusion The simple,rapid and reliable method can be used for the quality control of Xincang Granules.

关 键 词：辛苍颗粒 质量标准 黄芩苷 薄层色谱法 高效液相色谱法 

分 类 号：R914[医药卫生—药物化学]