miR-29b在结直肠癌组织中的表达及其临床意义  

Expression of microRNA-29b in colorectal cancer and its relationship with clinicopathological features

作　　者：马肖[1] 袁伟[1] 唐万燕[1] 马洁[1] 

机构地区：[1]国家癌症中心北京协和医学院中国医学科学院肿瘤医院分子肿瘤学国家重点实验室,100021

出　　处：《中华肿瘤杂志》2016年第6期430-433,共4页Chinese Journal of Oncology

基　　金：科技北京百名领军人才培养工程（Z13110700513001）;北京市科技新星计划（Z13110700413066）

摘　　要：目的检测结直肠癌组织和癌旁组织中miR．29b的表达水平，并分析其与患者临床病理特征和预后的关系。方法采用实时定量PCR方法检测202例结直肠癌组织和癌旁组织中miR．29b的表达水平，分析其与结直肠癌患者临床病理特征和预后的关系。结果miR-29b在结直肠癌组织和相应癌旁组织中的相对表达量分别为-7．761±0．115和-7．150±0．137，其在结直肠癌组织中的表达量明显降低（P〈0．01）。miR-29b在有淋巴结转移的结直肠癌组织和无淋巴结转移的结直肠癌组织中的相对表达量分别为-7．528＋0．158和-7．988±0．164，其在有淋巴结转移的结直肠癌组织中的表达量明显升高（P〈0．05）。miR-29b在I～Ⅱ期、Ⅲ期和Ⅳ期结直肠癌组织中的相对表达量分别为-8．096±0．157、-7．592±0．165和-6．603±0．468，其表达量呈逐渐升高的趋势，且差异有统计学意义（P〈0．05）。单因素Cox回归分析显示，淋巴结转移、肿瘤分化程度和TNM分期与结直肠癌患者的预后有关（均P〈0．001）。多因素Cox回归分析显示，肿瘤分化程度和TNM分期是影响结直肠癌患者预后的独立因素（均P〈0．001）。结论结直肠癌组织中miR-29b的表达低于癌旁组织。miR-29b的表达与结直肠癌的淋巴结转移和TNM分期有关。miR-29b有可能作为提示结直肠癌淋巴结转移及恶性进展的潜在标志物。Objective The aim of this study was to detect the expression level of miR-29b in colorectal cancer （CRC） tissues and to analyze its relationship with clinicopathological features and prognosis. Methods The expression of miR-29b was detected by real-time quantitative PCR in 202 colorectal cancer tissues and adjacent coloreetal tissues. Statistical analysis of the results was conducted using SPSS 16.0 software. Results The expression of miR-29b in colorectal cancer tissues and adjacent colorectal tissues was -7.761：t：0.115 and -7.150~0.137, respectively, with a significant difference between the two groups （P〈0.01）. Comparing colorectal cancer tissues with lymph node metastasis with those without lymph node metastasis, the expression of miR-29b （-7.528~0.158 vs. -7.988~0.164） was significantly increased （P〈0.05）. In addition, the expression of miR-29b in C RC according to TNM stage was -8.096~ 0.157 in stage I / II ,-7.592＋0.165 in stage m and -6.603＋0.468 in stage 1V patients, showing a gradual increase depending on clinical staging （P 〈 0.05 ）. Univariate Cox regression analysis indicated that lymph node metastasis, differentiation degree and TNM stage were significantly related to the postoperative survival of colorectal cancer patients （P〈 0.001 ）. Multivariate Cox regression analysis indicated that differentiation degree and TNM stage were independent factors impacting the survival of colorectal cancer patients （P〈 0.001 ）. Conclusions The expression level of miR-29b is reduced in colorectal cancer tissues compared with that in the adjacent colorectal tissues. High expression level of miR-29b is associated with lymph node metastasis and TNM stage of CRC. miR-29b may be a potential marker indicating colorectal cancer metastasis and malignant progression.

关 键 词：结直肠肿瘤 微小RNA29b 肿瘤转移 肿瘤分期 预后 

分 类 号：R735.34[医药卫生—肿瘤]