30个马铃薯卷叶病毒CP基因序列分析  

Analysis on CP Gene Sequences of Thirty Potato Leaf Roll Virus

作　　者：韩树鑫[1] 张俊华[1] 白艳菊[1,2] 张威[1,2] 高艳玲[1,2] 范国权[2] 张抒[2] 申宇[2] 

机构地区：[1]东北农业大学,黑龙江哈尔滨150030 [2]黑龙江省农业科学院植物脱毒苗木研究所,黑龙江哈尔滨150086

出　　处：《山东农业大学学报（自然科学版）》2016年第3期353-358,共6页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：现代农业产业技术体系专项资金资助(CARS-10-P14)

摘　　要：通过对含有PLRV的不同样品RT-PCR扩增,成功地克隆了PLRV CP基因,经比对样品的CP基因序列,核酸一致率为99.55%,仅发现了来自于广东的样品与其它样品有5个碱基的差异。试验样品与国内已报道的PLRV CP基因进行进化分析,不同地区的PLRV CP基因可分为A,B组,且有区域性特征。来自广东、云南及贵州的样品在两组中均有分布,但来自于内蒙古的样品仅聚类在A组。而中国样品与其他国家PLRV CP基因相比较,经进化树分组后,主要分为S1和S2两组,其中S1组中,包含了所有来自于我国的样品和绝大多数其它样品,且无规律可循;而S2组中的样品仅只来自于埃及和波兰。总体上,PLRV CP序列的差异仍然较小,因此,目前PLRV病毒的种群基因较稳定。We used RT-PCR to amplify the different samples with PLRV and successfully cloned PLRV CP gene by means of comparing CP gene sequence of samples to discover 99.55% of the nucleic acid concordance rate, there was only five bases difference between the sample from Guangdong and others. We had analyzed the test samples and other recorded PLRV CP genes in China and divided them from different districts into Group A, Group B. The results showed that samples from Guangdong, Yunnan and Guizhou distributed in two groups but only the samples from Inner Mongolia cluster in Group A.The PLRV CP genes from Chinese samples compared with other countries to be divided into Group S1 and Group S2. In Group S1, there were unordered samples from China and most of other countries; in Group S2, there were only samples from Egypt and Poland. In one word, there were a little difference in PLRV CP sequences so that at present the gene of the PLRV population was stable.

关 键 词：马铃薯 卷叶病毒 CP基因 序列分析 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治] Q943.2[农业科学—植物保护]