桦褐孔菌发酵液中三萜化合物和多糖的微波辅助双水相萃取  

Microwave-assisted aqueous two-phase system extraction of triterpenoids and polysaccharides in Inonotus obliquus fermentation broth

作　　者：刘琳[1] 宋丽敏[2] 

机构地区：[1]青岛农业大学生命科学学院山东省应用真菌重点实验室,山东青岛266109 [2]青岛农业大学农学与植物保护学院山东省植物病虫害综合防控重点实验室,山东青岛266109

出　　处：《食品与发酵工业》2016年第5期246-252,共7页Food and Fermentation Industries

基　　金：青岛农业大学高层次人才科研基金(6631112308;6631115038)

摘　　要：采用微波辅助乙醇/K2CO3双水相提取技术实现一步操作提取分离桦褐孔菌发酵液中的三萜化合物和多糖。通过考察分配系数和回收率,探讨三萜化合物和多糖在体系中的分配行为。获得三萜化合物和多糖的最佳微波提取条件为:最佳相组成中,K2CO3的质量分数为25%,乙醇的质量分数为24%;固液比为1∶50;微波功率为540 W;微波时间为120 s。此条件下,三萜化合物的提取率为(99.22±0.68)%,多糖的提取率为(98.34±0.83)%,蛋白去除率为(94.67±0.74)%,菌丝体去除率为100%。该方法操作简单,三萜化合物和多糖提取率高、分离效果好,同时去除了菌丝体和蛋白,更加有利于产品的后续纯化。Microwave-assisted aqueous two-phase extraction technology was applied to the extraction and separation of the triterpenoids and polysaccharides in the fermentation broth of Inonotus obliquus. The system of ethanol /K2CO3 was selected,and the distribution behaviors of the triterpenoids and polysaccharides were studied by investigating partition coefficient and recovery rate. Taking ethanol / K2CO3 as extract reagent,microwave assisted extraction was optimized. The optimal extraction conditions were as follows： the phase composition was K2CO3 and ethanol with mass fraction of 25% and 24% respectively,the ratio of solid to liquid was 1∶ 50,microwave power was 540 W,microwave time was 120 s. Herein the yields of triterpenoids and polysaccharides were（ 99. 22 ± 0. 68） % and（ 98. 34± 0. 83） %,respectively. The removal ratio of protein and mycelia were（ 94. 67 ± 0. 74） % and 100%,respectively. The extraction and separation of the triterpenoids and polysaccharides were integrated into one step using this method to realize the removal of protein and mycelia simultaneously. This was beneficial to the following purification operation.

关 键 词：乙醇/K2CO3双水相 桦褐孔菌 三萜化合物 多糖 微波辅助提取 

分 类 号：TQ929[轻工技术与工程—发酵工程] O658.2[理学—分析化学]