污染土壤中重金属Cr吸附菌株的筛选与鉴定  

Screening and Identification of Strains Adsorbing Heavy Metal Chromium in Contaminated Soil

作　　者：李军[1] 杨卓[2] 纪宏伟[1] 门明新[1] 

机构地区：[1]河北农业大学资源与环境科学学院,河北保定071000 [2]中国环境管理干部学院,河北秦皇岛066004

出　　处：《湖北农业科学》2016年第9期2218-2221,2226,共5页Hubei Agricultural Sciences

基　　金：2013年度河北省科学技术研究与发展计划项目(13227504D)

摘　　要：以Cr污染土壤为菌源样品,富集培养与菌株分离后,采用二苯碳酰二肼分光光度法从中筛选Cr吸附菌株,共筛选分离出4株高效吸附重金属Cr的细菌菌株。通过形态观察、生理生化试验对4株菌株进行了种属鉴定,初步鉴定4株菌株均为芽孢杆菌(Bacillus)。Strains that adsorb chromium were screened from contaminated soil using biphenyl carbonyl 2 hydrazine color-ultraviolet spectrophotometer method after enrichment culture and separation strains. Finally,4 bacteria strains that showed efficient adsorption on heavy metal chromium were separated. The experiments of species identifications on 4 strains were carried out by morphological observation, physiological and biochemical experiments, the results showed that 4 strains were all Bacillus.

关 键 词：土壤污染 重金属Cr 吸附 菌株筛选 种属鉴定 

分 类 号：X53[环境科学与工程—环境工程]