阴离子交换色谱-积分脉冲安培检测法测定赣南脐橙中的氨基酸  

Determination of Amino Acids in Navel Orange by Anion Exchange Chromatography with Integrated Pulsed Amperometric Detection

作　　者：王晓芬[1] 陈美芬[1] 刘俊俊[1] 鄢爱平[2] 万益群[1,2] 

机构地区：[1]南昌大学化学学院,江西南昌330031 [2]南昌大学分析测试中心,江西南昌330047

出　　处：《分析科学学报》2016年第3期330-334,共5页Journal of Analytical Science

基　　金：江西省科技支撑计划重大项目(No.20133ACG70002);江西省科技支撑计划项目(No.20141BBG70093;20141BBF60047);国家科技支撑计划(No.2012BAK17B00)

摘　　要：建立了阴离子交换色谱-积分脉冲安培法测定脐橙中17种氨基酸的分析方法。样品用无水乙醇提取,经聚苯乙烯-二乙烯基苯型阳离子交换树脂（PS-DVB）003x7去除碳水化合物,采用AminoPac PA10保护柱（50×2mm）和AminoPac PA10分析柱（250×2mm）分离,积分脉冲安培检测器检测。所测17种氨基酸在一定浓度范围内与峰面积呈良好的线性关系,相关系数均大于0.995,检出限为0.01～0.19mg/L。在2.0mg/L和8.0mg/L两个添加水平的平均回收率在72.5%～130.5%之间,相对标准偏差在0.5%～10.1%范围。该法具有较好的准确度和精密度,基本能满足实际样品分析的要求。A method for the determination of 17 amino acids in navel orange by anion-exchange chromatography with integrated pulsed amperometric detection was explored.Amino acids were extracted from samples of navel orange with anhydrous ethanol and carbohydrates were eliminated by PS-DVB 003x7 resin.The target analyte was separated on a AminoPac PA10（50×2mm）guard column and AminoPac PA10（250×2mm）analytical column,and detected by integrate pulsed amperometric detection（IPAD）using an Au working electrode.The method had good linearity in the measured concentration range of amino acids with correlation coefficients more than 0.995.The detection limits were in the range of 0.01-0.19mg/L.When 2.0mg/L and 8.0mg/L standard solutions were spiked for recovery experiments,recoveries were 72.5%-130.5%and the relative standard deviation were 0.5%-10.1%.The method has good accuracy and precision,which can basically meet the requirement of the actual sample analysis.

关 键 词：脐橙 氨基酸 阴离子交换色谱 

分 类 号：O657.75[理学—分析化学]