正交试验优选人参皂苷Re转化为Rg_2发酵工艺  

Fermentation Technology Optimization of Ginsenoside Re Biotransformed into Ginsenoside Rg_2 by Orthogonal Test

作　　者：李亨[1] 范秀双[1] 吴迪[1] 王云华[1] 杨苗[1] 尹建元[1] 孟勤[1] 江崇波 王春红[1] 

机构地区：[1]吉林大学药学院,长春130021 [2]杭州宝积生物科技有限公司,杭州310000

出　　处：《特产研究》2016年第2期17-19,36,共4页Special Wild Economic Animal and Plant Research

摘　　要：优选人参皂苷Re转化为人参皂苷Rg_2最佳发酵工艺条件,计算人参皂苷Rg_2转化产率。采用HPLC法,从发酵时间、发酵温度、复合菌添加量3方面,以人参皂苷Rg_2转化产率为评价指标,优选人参发酵条件,并进行相关性分析。人参发酵工艺为酶解温度60℃、加酶量10%、发酵时间10d。采用正交实验优选人参发酵工艺条件,该实验可以为人参皂苷Rg_2的工业化生产提供新的途径。Fermentation technology optimization of ginsenoside Re biotransformed into ginsenoside Rg_2 by orthogonal test and calculate yield of ginsenoside Rg2; Useing fermation time,fermation temperature,added content of composite enzymatic as model by HPLC,to optimization ginsenoside fermentation condition and correlation analysis; Ginsen fermentation technology was fermation temperature 60℃,added content of composite enzymatic 10%,fermation time 10 days; To apply the fermentation technology optimization of ginsenoside,it is the new way available for the ginsenoside Rg2 industrialized production.

关 键 词：人参皂苷RE 人参皂苷RG2 复合菌 正交实验 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]