508例恶性肿瘤患者外周血淋巴细胞亚群分析  

Analysis of the lymphocyte subsets of 508 cases of cancer patients

作　　者：刘云鹤[1] 贾英杰[1] 李文涛[1] 徐红达 于建春[1] 

机构地区：[1]天津中医药大学第一附属医院肿瘤科,300381 [2]天津中医药大学研究生院

出　　处：《国际肿瘤学杂志》2016年第6期405-408,共4页Journal of International Oncology

基　　金：国家自然科学基金（81273937）;高等学校博士学科点专项科研基金（20121210110010）;天津市抗癌重大攻关计划（12ZCDZSY15800）;天津市科委科技支撑计划（12CDZSY17000）;天津市卫生和计划生育委员会中医和中西医结合科研专项课题（2015090）

摘　　要：目的 研究肿瘤患者外周血淋巴细胞亚群变化特点.方法 收集天津中医药大学第一附属医院肿瘤患者508例（肺癌256例,胃癌152例,乳腺癌100例）,健康志愿者50例,采集入组人员外周血2 ml,应用BD流式细胞仪进行淋巴细胞亚群检测,所得数据采用SPSS 16.0软件进行统计分析.结果 淋巴细胞均数（比例）比较：肿瘤患者淋巴细胞数量降低,健康志愿者为5125 （34.2％）、乳腺癌为3642（24.3％）、胃癌为3178（21.2％）、肺癌为2895（19.3％）,依次降低.淋巴细胞亚群总异常病例数分别为肺癌218例（85.2％）、胃癌133例（87.5％）、乳腺癌88例（88.0％）;T细胞亚群异常病例数为肺癌83例（32.4％）、乳腺癌32例（32.0％）、胃癌44例（28.9％）;CD4 ＋/CD8＋异常病例数为肺癌185例（72.3％）、胃癌108例（71.1％）、乳腺癌84例（84.0％）;自然杀伤细胞亚群异常病例数为肺癌32例（12.5％）、胃癌22例（14.5％）、乳腺癌16例（16.0％）;B细胞亚群异常病例数肺癌38例（14.8％）、胃癌52例（34.2％）、乳腺癌12例（12.0％）.与健康志愿者相比,肺癌患者CD19＋％（12.8±5.0∶11.5±5.7,t=3.006,P=0.003）降低;胃癌患者CD4＋％ （39.2 ±7.7∶35.3±7.6,t=2.315,P=0.023）降低,CD19＋％ （12.8±5.0∶8.9±4.2,t=3.302,P=0.010）降低;乳腺癌患者CD8＋％（24.0±8.1∶29.1 ±13.0,t=2.019,P=0.047）升高.结论 肿瘤患者淋巴细胞数量减少,淋巴细胞亚群异常率增高,不同肿瘤患者淋巴细胞亚群异常变化表现出不同的特点.Objective To study the characteristics of peripheral blood lymphocyte subsets in patients with lung cancer,gastric cancer and breast cancer.Methods Five hundred and eight patients with cancer from the First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine （256 cases with lung cancer,152 cases with gastric cancer,100 cases with breast cancer）,and 50 healthy volunteers were collected.Two ml peripheral blood were obtained from these cases.The distribution of lymphocyte subsets in peripheral blood was measured by flow cytometry in these cases.The data were analyzed using SPSS 16.0 software.Results The number of lymphocytes of cancer patients was decreased,the healthy volunteers was 5125,breast cancer patient was 3642,gastric cancer patient was 3178,lung cancer patient was 2895.The case of outliers of lymphocyte subsets classified based on the three kinds of cancer was 218 （85.2%,lung cancer）,133 （87.5%,gastric cancer）,88 （88.0%,breast cancer）.The case of outliers of T-lymphocyte subsets classified based on the three kinds of cancer was 83 （32.4%,lung cancer）,32 （32.0%,breast cancer）,44 （28.9%,gastric cancer）.The case of outliers of CD4 ＋/CD8 ＋ classified based on the three kinds of cancer was 185 （72.3%,lung cancer）,108 （71.1%,gastric cancer）,84 （84.0%,breast cancer）.The case of outliers of natural killer-lymphocyte subsets classified based on the three kinds of cancer was 32 （12.5%,lung cancer）,22 （14.5%,gastric cancer）,16 （16.0%,breast cancer）.The case of outliers of B-lymphocyte subsets classified based on the three kinds of cancer was 38 （14.8%,lung cancer）,52 （34.2%,gastric cancer）,12 （12.0%,breast cancer）.Compared to healthy subjects,the CD19＋% of patients with lung cancer was decreased （12.8 ± 5.0 vs.11.5 ± 5.7,t =3.006,P =0.003）;the CD4 ＋ % of patients with gastric cancer was decreased （39.2 ±7.7 vs.35.3 ± 7.6,t =2.315,P =0.023）;the CD19 ＋ % of patients with gastric cancer was decreased （12.8 ± 5.

关 键 词：肺肿瘤 胃肿瘤 乳腺肿瘤 淋巴细胞亚群 

分 类 号：R730.43[医药卫生—肿瘤]