饥饿及恢复喂食对花鲈肠道菌群多样性的影响  

Effects of fasting and refeeding on Lateolabrax japonicus intestinal microflora diversity

作　　者：潘艳艳[1] 顾晓英[1] 张德民[1] 钱云霞[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《水产学报》2016年第5期776-784,共9页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2012AA092001);宁波大学"水产"浙江省重中之重开放基金(xkzsc1513)~~

摘　　要：为了研究饥饿及恢复摄食对花鲈肠道壁及内容物微生物菌群的影响,实验运用末端限制性片段长度多态性(T-RFLP)技术分析了花鲈经2周饥饿,恢复喂食1周和恢复喂食2周后肠道壁及其内容物菌群特征及多样性的变化。结果显示:饥饿会导致花鲈肠道壁细菌群落发生明显变化,引起差异的主要细菌为T-RFs 496、437、450、155 bp等所代表菌;经2周饥饿,肠壁T-RF 496 bp大肠杆菌(Escherichia)相对丰度从实验开始的43.11%±3.95%(C0肠壁组)下降为21.25%±9.97%(S2R0肠壁组),细菌多样性指数H′、E′和1/D均增大;恢复喂食2周后,肠道壁菌群结构逐渐恢复,T-RF 496 bp大肠杆菌的相对丰度逐渐上升到55.49%±8.37%(S2R2组),3个多样性指数均减小至原有水平;花鲈肠道壁和内容物的菌群有较大不同,但是两者的主要细菌类群都是变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)和拟杆菌门(Bacteroidetes),其中,γ-变形菌纲是花鲈肠道的最主要细菌群。本研究为进一步阐述消化道微生物功能奠定了基础,也为海水鱼类肠道菌群研究提供基础数据。Microflora in fish digestive tract play important roles in digestion, absorption, immunity and organ development. In this study, terminal restriction fragment length polymorphism（T-RFLP） technique was employed to examine the changes in structure of the intestinal microbiota of L. japonicus after fasting and refeeding. The results showed that microflora structure of Lateolabrax japonicus intestinal wall changed after 2 weeks of fasting.The main T-RFs that caused the microflora structure differences among fasting group, refeeding groups and control groups were T-RFs 496 bp, 437 bp, 450 bp and 155 bp.Especially, after 2 weeks of fasting, the relative abundance of T-RF 496 bp（Escherichia） reduced to 21.25%±9.97% from 43.11%±3.95% of the beginning（C0）and the bacterial diversity index（H′, E′ and 1/D） of intestinal wall increased significantly compared with control groups. Bacterial diversity index（H′, E′ and 1/D） of intestinal wall were decreased and the microflora structure was recovered gradually with the refeeding. Meanwhile, the relative abundance of T-RF 496 bp gradually increased to55.49%±8.37%.The results also indicated that the microflora structure of intestinal walls was different from that of intestinal contents, but their main phyla were the same, namely, Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes.

关 键 词：花鲈 饥饿 恢复喂食 肠道菌落 末端限制性片段长度多态性技术 

分 类 号：Q938.8[生物学—微生物学] S965[农业科学—水产养殖]