哺乳期大鼠乳腺组织磷脂酰肌醇-3激酶-蛋白激酶B信号通路对摄碘功能的影响  

Effects of phosphatidylinositol-3 kinase-protein kinase B signaling pathway on iodine-intake ability of lactating rats

作　　者：文达[1] 薛靓[1] 刘丽香[1] 王丹丹[1] 闫英男[1] 申红梅[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心碘缺乏病防治研究所国家卫生计生委病因流行病学重点实验室(23618504)、黑龙江省普通高校病因流行病学重点实验室,哈尔滨150081

出　　处：《中华地方病学杂志》2016年第6期395-400,共6页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81273012）;高等学校博士科学点专项科研基金（20122307110010）

摘　　要：目的观察不同碘营养水平下大鼠乳腺组织磷脂酰肌醇-3激酶（P13K）、蛋白激酶B（AKT）、钠碘转运体（NIS）mRNA和蛋白的表达，以及血清胰岛素样生长因子I（IGF-1）的变化，探讨该通路在哺乳期乳腺摄碘过程中所起到的作用。方法Wistar大鼠130只（雌鼠100只，雄鼠30只），按体质量采用随机数字表法将雌鼠分为5组：①对照组（NI组），普通饲料＋含碘50μg／L去离子水；②低碘1组（L11组），低碘饲料＋去离子水；⑧低碘2组（L12组），低碘饲料＋含碘5μg／L去离子水；④高碘1组（H11组），普通饲料＋含碘3000μg／L去离子水；⑤高碘2组（H12组），普通饲料＋含碘10000μg／L去离子水。每组20只，饲养3个月后，将雌鼠与雄鼠合笼，交配后，取出雄鼠，每只雌鼠单独喂养，在产后10d时，采集尿样，测定哺乳期大鼠尿碘，处死后取哺乳期大鼠的血液、乳腺组织。酶联免疫吸附法测定哺乳期大鼠血清IGF-1水平；实时荧光定量PCR法检测乳腺P13K、AKT、NISmRNA表达；蛋白印迹法检测乳腺P13K、总AKT、磷酸化AKT（p—AKT）和NIS蛋白表达。结果L11、L12组哺乳期大鼠尿碘中位数（3．16、6．36μg／L）明显低于NI组（162．59μg／L），H11、H12组（2356．27、11507．29μg／L）明显高于NI组，差异均有统计学意义（P均〈0．01）。与NI组[（8．84±2．12）μg／L]比较，L11、L12组哺乳期大鼠血清IGF-1含量[（13．30±2．37）、（10．90±1．92）μg／L]明显升高（P均〈0．01）。实时荧光定量PCR法检测结果显示．哺乳期大鼠乳腺组织NIS、AKT、P13KmRNA表达组间比较差异有统计学意义（F=14．916、36．477、14．994，P均〈0．01）。其中NISmRNA表达在L11、L12组（0．75±0．40、0．89±0．51）明显高于NI组（0．53±0.31），H12组（0．30±0．24）明显低于NI组（P〈0．05或〈0．01）；AKTmRNA表达在U1、U2组（0．90±0．19、0．64±Objective To observe the variation of phosphatidylinositol-3 kinase （PI3K）, protein kinase B （AKT）, sodium iodide symporter （NIS） mRNA and protein expression in rat mammary tissues and serum insulin growth factor I （IGF-1） under different iodine nutrition levels, and to study the role of PI3K-AKT signaling pathway in the process of mammary gland intaking iodine during lactation period. Methods Totally 130 Wistar rats （100 female rats, 30 male rats） were randomly divided into five groups with 20 female rats in each group： （1）control group （NI）： was feed with normal diet and iodine content 50 μg/L in deionized water; （2）low iodine group 1 （LI1 group）： was feed with low iodine diet and deionized water;, （3）low iodine group 2 （LI2）： was feed with low iodine diet and iodine content 5 μg/L in deionized water; （4）high iodine group 1 （HI1 group）： was feed with normal diet and iodine content 3 000 μg/L in deionized water; （5）high iodine group 2 （HI2）： was feed with normal diet and iodine content 10 000 μg/L in deionized water. After feeding for 3 months, females were mated with male rats, then male rats were taken out and every female rat was feed individually. Urinary iodine level of rats in lactation period 10 days after giving birth was tested. Blood and mammary tissue samples of rats in lactation period were taken after killing them. Enzyme linked immunosorbent assay （ELISA） was used to detect serum IGF-1 level, real-time fluorescence quantification PCR to detect the mRNA expression of mammary gland PI3K, AKT and NIS, Western blotting to detect mammary gland PI3K, total AKT, phosphorylation AKT （p-AKT） and NIS protein expression. Results The medians urinary iodine of lactation period rats in LI1 and LI2 （3.16, 6.36 μg/L） were significantly lower than that in NI group （162.59 μg/L）, and were significantly higher in HI1 and HI2 （2 356.27, 11 507.29 μg/L） than that in NI group. The differences were statistically significant

关 键 词：磷脂酰肌醇-3激酶-蛋白激酶B信号通路 钠碘转运体 哺乳期大鼠 乳腺 碘 

分 类 号：R151[医药卫生—营养与食品卫生学]