HPLC法测定复合维生素B片维生素B_1、维生素B_2、烟酰胺的含量及含量均匀度  

Determination of Content and Content Uniformity of Vitamin B_1,Vitamin B_2 and Niacinamide in Compound Vitamin B Tablets by HPLC

作　　者：陈华龙 刘旺培 

机构地区：[1]广东韶关市食品药品检验所,韶关512028

出　　处：《海峡药学》2016年第2期63-65,共3页Strait Pharmaceutical Journal

摘　　要：目的建立高效液相色谱法测定复合维生素B片维生素B1、维生素B2、烟酰胺的含量及含量均匀度。方法采用色谱柱：Diamonsil C18（250mm×4.6mm,5μm）,流动相：0.005mol·L-1庚烷磺酸钠溶液（含0.5%冰醋酸和0.05%三乙胺）-甲醇（70∶30,V/V）;检测波长为261nm,流速为1.0m L·min-1,进样量：20μL。结果维生素B1、维生素B2、烟酰胺浓度分别在2.65~66.2μg·m L-1、1.19~30.15μg·m L-1、7.83~196.2μg·m L-1范围内与峰面积线性关系良好,平均回收率均大于98%,RSD均小于1.0%。结论本方法简单、精确、可靠,可作为合维生素B片的质量控制方法。OBJECTIVE To establish HPLC determination of compound vitamin B,vitamin B1,vitamin B2,niacinamide content and content uniformity. METHODS The chromatographic column： Diamonsil C18（ 250 mm ×4. 6mm,5μm）,mobile phase： 0. 005mol·L- 1heptane sodium sulfonate solution（ including 0. 5% glacial acetic acid and 0. 5% triethylamine）-methanol（ 70 ∶ 30,V / V）; Detection wavelength of 261 nm,flow rate of 1. 0m L · min- 1,sample quantity： 20μL. RESULTS The concentration of vitamin B1,vitamin B2,and nicotinamide has a good linear relationship in the range of 2. 65 ~ 66. 2μg·m L- 1,1. 19 ~ 30. 15μg·m L- 1,7. 83 ~ 196. 2μg·m L- 1respectively,the average recovery were greater than 98%,RSD were less than 1. 0%. CONCLUSION This method is simple,accurate,reliable and can be used as the quality control of vitamin B slice method.

关 键 词：高效液相色谱法 复合维生素B片 维生素B1 维生素B2 烟酰胺 含量 含量均匀度 

分 类 号：R927.2[医药卫生—药学]