印度尼西亚卡利安达岛近岸热泉微生物和氢酶基因的多样性  

Diversity of microbe and hydrogenase genes from a coastal hot spring of Kalianda,Indonesia

作　　者：何培青[1] Dewi Seswita Zilda 李江[1] 张学雷[1] 崔菁菁[3] 白亚之[3] Gintung Patantis Ekowati Chasanah 

机构地区：[1]国家海洋局第一海洋研究所海洋生态研究中心,山东青岛266061 [2]海洋与渔业局海洋与渔业研究与发展司海洋和渔业产品加工和生物技术研究中心,印度尼西亚雅加达40115 [3]国家海洋局第一海洋研究所海洋沉积与环境地质国家海洋局重点实验室,山东青岛266061

出　　处：《海洋学报》2016年第6期119-129,共11页

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2011T04);国家海洋局海洋生物活性物质与现代分析技术重点实验室开放课题(MBSMAT-2015-06;MBSMAT-2011-03;MBSMAT-2012-2)

摘　　要：研究对2011年7月采自印度尼西亚卡利安达岛近岸热泉的样品,进行了细菌和古菌16SrRNA基因和细菌氢酶基因克隆文库的构建、序列测定和多样性分析。结果表明:热泉系统的细菌和古菌由16个门组成,Proteobacteria为热液流体和沉积物的优势类群,丰度分别为59.5%和73.3%,Cyanobacteria为菌苔的优势类群(丰度,56.5%);具有氨氧化作用的Crenarchaeota为古菌的优势类群。热泉系统中,70%以上的种类与海洋细菌的亲缘关系最近,说明该系统具有海洋特性;约20%的种类与陆地热泉中嗜热或中等嗜热细菌的亲缘关系最近,这些种类可能来源于高温地层内部,并参与了铁氧化还原、氢氧化、硫氧化和硝酸还原等过程。热泉系统中,NAD(P)-关联的双向NiFe-氢酶基因和FeFe-氢酶基因的组成分布也受到温度和盐度分布变化的影响。研究为揭示浅海热液系统提供理论参考。During July,2011,samples were collected from a coastal hot spring of Kalianda,Indonesia.Clone libraries of 16 SrRNA genes of bacteria and archaea,and hydrogenase genes were constructed.The sequences were determined,and the diversity were also analyzed.The results demonstrated that the coastal hot spring hosted 16 phyla.Proteobacteria was dominant in hydrothermal fluid and sediment,accounting for 59.5% and 73.3%,respectively.Cyanobacteria was the most abundant in mat with abundance of 56.5%.Ammonia oxidizing Crenarchaeota was predominant in archaea.More than 70% of the species were most related to marine microbes,indicating the marine property of this system.About 20% of the species were most related to the thermophilic or moderate thermophilic microbes from terrestrial hot springs.These species might originate from the hyperthermal subfloor and involve in the process of iron oxidation and reduction,hydrogen oxidation,sulfur oxidation and nitrate reduction.The composition and distribution of NAD（P）-linked bidirectional NiFe-hydrogenase and FeFe-hydrogenase genes were also shaped by temperature and salinity.Our research provided an insight into the shallow-sea hydrothermal system.

关 键 词：印度尼西亚卡利安达岛 近岸热泉 16SrRNA 氢酶基因 

分 类 号：Q938[生物学—微生物学]