黑龙江省规模化猪场种猪RYRⅠ基因多态性检测分析  

Analysis of Pig RYRⅠ Gene Polymorphism in Heilongjiang Large Scale Pig Farm

作　　者：张冬杰[1] 曹越[2] 何鑫淼[1] 汪亮[1] 刘娣 

机构地区：[1]黑龙江省农业科学院畜牧研究所,黑龙江哈尔滨150086 [2]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《黑龙江农业科学》2016年第6期75-78,共4页Heilongjiang Agricultural Sciences

基　　金：黑龙江省科技厅省院科技合作专项资助项目(YS14B03)

摘　　要：猪肉品质一直以来都是种猪培育过程中的一个关键指标。兰尼定Ⅰ型受体基因（RYRI）是一个有效的影响猪肉品质的候选基因，它的第615位氨基酸突变是造成猪发生应激综合症，进而出现PSE肉的主要原因。因此，国内外很多猪场都对该基因进行了清除。为加速优质种猪选育进程，选择了黑龙江省5家规模化猪场，对猪场内后备种猪的RYRⅠ基因进行了基因分型与检测。结果表明：有3家猪场的后备种猪中有nn型个体，但数量非常少；5家猪场内均有杂合型个体，占到所检个体的10％～20％。说明黑龙江省种猪企业对RYRⅠ基因的清除工作还有待加强，猪肉品质提升还有很大空间。Pork quality is always a key indicator in the process of pig breeding. Ryanodine receptor type 1 gene is an effective candidate genes influence the quality of pork. Its 615^th amino acid mutation is the main cause of porcine stress syndrome and PSE meat. Therefore,this gene was cleaned in many pig farm of domestic and overseas. The polymorphisms of RYRⅠ of the reserved boar in 5 large scale pigs were detected by PCR-RFLP. The results showed that 3 pig farms had nn genotype individual, but the number was very little. The Nn genotype individual existed in 5 pig farms and occupied 10-20%. These results indicated that the clean work of RYRⅠ was remain to be strengthened and there was still a great space to improve the pork quality.

关 键 词：民猪 大白猪 RYR1基因 多态性检测 

分 类 号：S828[农业科学—畜牧学]