miR-124抑制食管鳞癌细胞TE-1增殖及侵袭的研究  

microRNA-124 inhibits proliferation and invasiveness of esophageal squamous cell carcinoma cell line TE-1

作　　者：张玉虹[1,2] 李皓[3] 叶婷[3] 高飞[2] 王强强[1,2] 刘阳晨[2] 

机构地区：[1]蚌埠医学院研究生部,安徽蚌埠233000 [2]泰兴市人民医院放疗科,江苏泰兴225400 [3]泰兴市人民医院检验科,江苏泰兴225400

出　　处：《实用肿瘤杂志》2016年第3期232-237,共6页Journal of Practical Oncology

摘　　要：目的分析微RNA-124(microRNA-124,miR-124)在食管癌组织中的表达情况,并探讨miR-124在食管鳞癌增殖及侵袭中的作用及机制。方法采用实时荧光定量RT-PCR检测miR-124在18例食管癌组织(Ⅰ期6例,Ⅱ期8例,Ⅲ期4例,Ⅳ期0例)及相应的癌旁组织中的表达情况。在食管癌细胞TE-1中转染miR-124 mimic,采用细胞集落形成实验、细胞计数试剂盒-8(cell counting kit-8,CCK-8)、细胞划痕实验检测miR-124过表达对食管癌细胞增殖及侵袭的影响。通过Targetscan预测细胞周期蛋白依赖性激酶(cyclin-dependent kinases 4,CDK4)为miR-124的直接靶基因,并用荧光素酶报告基因实验及蛋白质印迹法实验进行验证。结果食管癌组织miR-124的表达水平较癌旁正常组织明显下降(P<0.01)。通过转染miR-124 mimics使TE-1细胞过表达miR-124,过表达miR-124后TE-1细胞增殖及侵袭能力明显下降(P<0.01)。结论 miR-124在食管癌组织中低表达,miR-124可能通过调控CDK4表达来影响食管癌细胞TE-1增殖及侵袭能力。Objective To investigate the expression of microRNA-124（ miR-124） in human esophageal squamous cell carcinoma（ ESC） tissues,and the effects of miR-124 on ESC cell proliferation and invasion. Methods The expression of miR-124 in 18 samples of ESC tissues（ stage Ⅰ： 6 cases,stage Ⅱ： 8,stage Ⅲ： 4,stage Ⅳ： 0） and tumor adjacent tissues were determined by quantitative real-time RT-PCR. Human ESC TE-1 cells were transfected with miR-124 mimics,and the effects of miR-124 on proliferation and invasiveness of ESC cells were analyzed by colony survival assay,cell counting kit-8（ CCK-8） and wound-healing assay. The target of miR-124,cyclin-dependent kinase-4（ CDK4）,as predicted by Targetscan was verified with luciferase assay and Western blot. Results The expression level of miR-124 in ESC was significantly lower than that in tumor adjacent tissues（ P〈0. 01）. The expression of miR-124 in TE-1 cells transfected with miR-124 mimics was significantly increased,while cell proliferation and invasiveness were decreased（ P〈0. 01）. Conclusion The expression of miR-124 is down-regulated in human esophageal cancer tissues,and it may regulate proliferation and invasiveness via CDK4 in ESC TE-1 cells.

关 键 词：食管肿瘤/病理学 癌 鳞状细胞/病理学 微RNAs/药理 细胞周期蛋白依赖激酶4 细胞增殖 肿瘤侵润 

分 类 号：R735.1[医药卫生—肿瘤] R730.23[医药卫生—临床医学]