GDF11对肝细胞癌SMMC-7721细胞增殖能力及顺铂敏感度的影响  

GDF11 is Involved in Human Hepatic Carcinoma Cells SMMC-7721 Proliferation and Sensitivity to DDP

作　　者：张菊萍[1] 史业辉[1] 贾勇圣[1] 周立艳[1] 佟仲生[1] 

机构地区：[1]天津医科大学肿瘤医院乳腺肿瘤内科,国家肿瘤临床医学研究中心,乳腺癌防治教育部重点实验室,天津市肿瘤防治重点实验室,天津300060

出　　处：《肿瘤防治研究》2016年第6期459-462,共4页Cancer Research on Prevention and Treatment

摘　　要：目的研究GDF11(growth differentiation factor 11)过表达对肝细胞癌SMMC-7721细胞体外增殖能力及顺铂敏感度的影响。方法前期成功构建GDF11过表达的SMMC-7721细胞,同时以空载体感染细胞组及野生型细胞组为对照。CCK-8法检测细胞增殖能力,平板克隆形成实验检测细胞克隆形成能力。CCK-8法检测GDF11过表达对细胞顺铂药物敏感度的影响。结果 Western blot结果验证,已成功构建GDF11过表达的SMMC-7721细胞。GDF11过表达可明显抑制SMMC-7721细胞的体外增殖能力,在96、120及144 h时抑制作用最显著(P<0.05),且实验组细胞较对照组克隆形成能力明显减弱(P<0.05)。GDF11过表达能够增强肝细胞癌SMMC-7721细胞对顺铂的敏感度,顺铂浓度取20μg/ml及40μg/ml时,与对照组相比差异有统计学意义(P<0.05)。结论肝细胞癌SMMC-7721细胞过表达GDF11蛋白后,其体外增殖及克隆形成能力明显降低,对化疗药物顺铂的敏感度增强。Objective To explore the influence of growth differentiation factor 11（GDF11） overexpression on hepatic carcinoma cells SMMC-7721 proliferation ability and sensitivity to DDP in vitro. Methods Previous study had successfully built SMMC-7721 cells with GDF11 over-expression. Meanwhile, empty vector infected cells and wild-type cells were treated as controls. Cells proliferation ability was examined by CCK-8 assay, and the proliferation curve was calculated. Colony formation assay was adopted to evaluate colony formation ability. The SMMC-7721 cells were treated with various concentrations of DDP, then the cell viability was measured using CCK-8 assay. Results Western blot showed SMMC-7721 cells with GDF11 over-expression had successfully built. The overexpression of GDF11 could significantly inhibit the proliferation of SMMC-7721 cells in vitro, and the effect were significant at 96, 120, 144h（P〈0.05）. Meanwhile GDF11 over-expression group had lower ability of colony formation than control groups（P〈0.05）. The over-expression of GDF11 made SMMC-7721 cells more sensitive to DDP, and the effect were significant at 20 and 40μg/ml DDP（P〈0.05）.Conclusion The over-expression GDF11 could inhibit the proliferation and colony formation abilities of SMMC-7721 cells, and enhance its sensitivity to DDP.

关 键 词：肝细胞癌 GDF11 增殖 顺铂 敏感度 

分 类 号：R735.7[医药卫生—肿瘤]