热敏脂质体联合微波热疗对HeLa细胞的增殖抑制作用  

Inhibitory Effect of Thermosensitive Liposomes Combined with Microwave Thermotherapy on Human Cervical Carcinoma Cells HeLa

作　　者：王雪莉[1] 马红岩[1] 

机构地区：[1]商丘医学高等专科学校附属医院妇产科,商丘476005

出　　处：《肿瘤防治研究》2016年第6期468-472,共5页Cancer Research on Prevention and Treatment

摘　　要：目的构建阿霉素热敏脂质体(DOX-TSLs),并研究其与微波热疗联合对人宫颈癌He La细胞的治疗作用。方法使用逆向蒸发法制备DOX-TSLs;检测其包封率及体外热敏释放情况;MTT法检测He La细胞在化疗、热疗及联合治疗时的存活率;流式细胞术检测联合治疗时的细胞凋亡情况。结果成功构建DOX-TSLs,平均粒径为(190.7±4.8)nm,包封率88.5%;体外累积释放率实验结果表明,DOX-TSLs可在微波热疗条件下迅速释放药物,30 min药物累积释放率达85%以上;DOX-TSLs可在6 h内完全进入肿瘤细胞内部;DOX-TSLs与微波热疗联合可有效抑制肿瘤细胞增殖;与热疗联合能促进细胞凋亡,联合治疗时细胞凋亡率为(62.2±2.3)%。结论 DOX-TSLs与微波热疗联合治疗人宫颈癌He La细胞具有明显协同作用,为宫颈癌的治疗模式提供了新的理论及实验依据。Objective To construct the thermos-sensitive liposomes loading with doxorubicin drug delivery system（DOX-TSLs） and investigate the effect of its combination with microwave thermotherapy on human cervical carcinoma cells He La in vitro. Methods Reverse evaporation method was used to prepare DOXTSLs; drug loading rate and in vitro release were detected; MTT method was used to detect the inhibition of He La cells treated with chemotherapy, thermotherapy and the combination; the apoptosis rate was detected by flow cytometry. Results DOX-TSLs drug delivery system was constructed successfully with the average particles size（190.7±4.8） nm and the efficiency 88.5%; the in vitro release detection revealed that the cumulative release rate could reach 85% with microwave thermotherapy at 30 min; DOX could be delivered into He La cells within 6 h by DOX-TSLs; the combination therapy of DOX-TSLs and microwave thermotherapy could inhibit cell proliferation effectively and significantly promote cell apoptosis which could be reach（62.2±2.3）%. Conclusion DOX-TSLs combined with microwave thermotherapy in the treatment of human cervical carcinoma cells He La have a synergistic effect. This therapy provides newly theoretical and experimental basis of human cervical carcinoma.

关 键 词：热敏脂质体 微波 热疗 联合治疗 

分 类 号：R737.33[医药卫生—肿瘤]