Enhancer activity of Hefitron in sericin-1 gene promoter from Bombyx mori  

作　　者：Ke Huang Chun-Feng Li Jie Wu Jun-Hong Wei Yong Zou Min-Jin Han Ze-Yang Zhou 

机构地区：[1]State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing [2]College of Forestry & Life Science, ChongqingUniversity of Arts & Sciences, Yongchuan [3]Laboratory of Animal Biology, Chongqing Normal University, Chongqing, China

出　　处：《Insect Science》2016年第3期396-405,共10页昆虫科学（英文版）

摘　　要：Sericin is a kind of water-soluble protein expressed specifically in the middle silk gland ofBombyx mori. When the sericin-1 gene promoter was cloned and a transgenic vector was constructed to express a foreign protein, a specific Helitron, Bmhel-8, was identified in the sericin-1 gene promoter sequence in some genotypes of Bombyx mori and Bombyx mandarina. Given that the Bmhel-8 Helitron transposon was present only in some genotypes, it could be the source of allelic variation in the sericin-I promoter. The length of the sericin-1 promoter sequence is approximately 1063 or 643 bp. The larger size of the sequence or allele is ascribed to the presence of Bmhel-8. Silkworm genotypes can be homozygous for either the shorter or larger promoter sequence or heterozygous, containing both alleles. Bmhel-8 in the sericin-1 promoter exhibits enhancer activity, as demonstrated by a dual-luciferase reporter system in BraE cell lines. Furthermore, Bmhel-8 displays enhancer activity in a sericin-1 promoter-driven gene expression system but does not regulate the tissue-specific expression of sericin-1.Sericin is a kind of water-soluble protein expressed specifically in the middle silk gland ofBombyx mori. When the sericin-1 gene promoter was cloned and a transgenic vector was constructed to express a foreign protein, a specific Helitron, Bmhel-8, was identified in the sericin-1 gene promoter sequence in some genotypes of Bombyx mori and Bombyx mandarina. Given that the Bmhel-8 Helitron transposon was present only in some genotypes, it could be the source of allelic variation in the sericin-I promoter. The length of the sericin-1 promoter sequence is approximately 1063 or 643 bp. The larger size of the sequence or allele is ascribed to the presence of Bmhel-8. Silkworm genotypes can be homozygous for either the shorter or larger promoter sequence or heterozygous, containing both alleles. Bmhel-8 in the sericin-1 promoter exhibits enhancer activity, as demonstrated by a dual-luciferase reporter system in BraE cell lines. Furthermore, Bmhel-8 displays enhancer activity in a sericin-1 promoter-driven gene expression system but does not regulate the tissue-specific expression of sericin-1.

关 键 词：Bmhel-8 ENHANCER PROMOTER regulation sericin-I 

分 类 号：Q754[生物学—分子生物学] Q78