Insight into the possible mechanism of the summer diapause of Delia antiqua （Diptera： Anthomyiidae） through digital gene expression analysis  

作　　者：You-Jin Hao Yu-Juan Zhang Feng-Ling Si Dan-Ying Fu Zheng-Bo He Bin Chen 

机构地区：[1]Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing, China

出　　处：《Insect Science》2016年第3期438-451,共14页昆虫科学（英文版）

基　　金：Acknowledgments This work was supported by Par-Eu Scholars Program, and The National Natural Science Foundation of China （31372265）, Coordinated Research Project of the International Atomic Energy Agency （18268/R0）, and National Key Program of Science and Technology Foun- dation Work of China （2015FY210300）. Conceived and designed the research： BC. Performed the experiments： FLS, DYE ZBH; Analyzed the data and wrote the paper： YJH, BC, YJZ.

摘　　要：The onion fly, Delia antiqua, is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons. However, little is known about its molecular regulation due to the lack of genomic resources. To gain insight into the possible mechanism of summer diapause （SD）, high-throughput RNA-Seq data were generated from non-diapause （ND） and SD （initial, maintenance and quiescence phase） pupae. Three pair-wise comparisons were performed and identified, 1380, 1471 and 435, and were significantly regulated transcripts. Further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation, cell cycle, carbon hydrate and lipid metabolism, innate immune and stress responses, various signalling transductions, ubiquitin-dependent proteosome, and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD. Global characterization oftranscriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD. Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.The onion fly, Delia antiqua, is a major underground agricultural pest that can enter pupal diapause in the summer and winter seasons. However, little is known about its molecular regulation due to the lack of genomic resources. To gain insight into the possible mechanism of summer diapause （SD）, high-throughput RNA-Seq data were generated from non-diapause （ND） and SD （initial, maintenance and quiescence phase） pupae. Three pair-wise comparisons were performed and identified, 1380, 1471 and 435, and were significantly regulated transcripts. Further analysis revealed that the enrichment of several functional terms related to juvenile hormone regulation, cell cycle, carbon hydrate and lipid metabolism, innate immune and stress responses, various signalling transductions, ubiquitin-dependent proteosome, and variation in cuticular and cytoskeleton components were found between ND and SD and between different phases of SD. Global characterization oftranscriptome profiling between SD and ND contributes to the in-depth elucidation of the molecular mechanism of SD. Our results also offer insights into the evolution of insect diapause and support the importance of using the onion fly as a model to compare the molecular regulation events of summer and winter diapauses.

关 键 词：Delia antiqua digital gene expression possible mechanism qPCR RNA-SEQ summer diapause 

分 类 号：Q968[生物学—昆虫学] Q786