Protective Effect of phytolacca Acinosa Polysaccharides Ⅰ(PAP-Ⅰ)on Hematopoiesis in Cyclophosphamide-treated and 60￣Co-irradiated Mice  

商陆多糖I对给环磷酰胺及^（60）Co照射小鼠造血功能的保护作用

作　　者：王洪斌[1] 郑钦岳[1] 孙盛川[1] 刘宝云 沈有安[1] 唐惠兰[1] 鞠佃文[1] 方军[1] 

机构地区：[1]上海第二军医大学药学院药理教研室,上海第二军医大学长征医院药局

出　　处：《Journal of Chinese Pharmaceutical Sciences》1995年第2期89-98,共10页中国药学（英文版）

摘　　要：The protective effect of a kind of purified polysaccharides extracted from Radix of Phytolacca acinosa Roxb,with a molecular weight of 10 KDa,on hematopoiesis was investigated.Average survival time of mice treated with cyclophosphamide (CY) 300 mg/kg once alone was 13.3 ± 7.2d(n=7) however,average survival time of mice treated with CY 300 mg/kg in com-bination with PAP-1 10 mg/kg,3 times/wk was 36.7± 16.4d(n=7,P<0.01).PAP-1,ip had benefi-cial effect on the recovery of the CY induced decrease of peripheral leukocyte number,and the nu-cleated bone marrow cell(BMC)number and[3￣H]TdR uptaken by BMC induced by rmGM-CSF in S180 bearing mice treated with CY,In mice,after the first ip treatment with CY 100 mg/kg on d7,the peripheral leukocyte number decreased on d9 and recovered to normal level about d13 to d15. Such recovery was accelerated by administrating PAP-1,10mg/kg, 3 times/wk.A significant in-crease of the activity to form colony in spleen(colony-forming unit in spleen, CFU-S_8, CUF-S12) in mice irradiated with 550 rad 6O￣Co γ-rays and an enhancement of proliferative response of BMC to rmGM-CSF treated with PAP-1,10mg/kg,3 times/wk, ip were observed.After PAP-1,10 mg/kg,ip once,a significant increase in the number of peripheral blood leukocytes and a rise in the serum of colony stimulating factor(CSF) were also confirmed.The types of CSF in serum were M-CSF and other hematopoietic growth factors,which were confirmed by using McAb of IL-3, GM-CSF and PcAb of M-CSF. These beneficial effects of PAP-1 on hematopoiesis may be related to its activityinducing CSFs and other hematopoietic growth factors and warrant further evaluation of its use-fulness.正常小鼠给商陆多糖Ｉ（ＰＡＰ─Ｉ），１０ｍｇ／ｋｇ每周３次，给药一周后，腹腔注射ｃｙｃｌｏｐｈｏｓｐｈａｍｉｄｅ（ＣＹ），３００ｍｇ／ｋｇ，小鼠的平均存活时间由１３．３±７．２ｄ延长至３６．７±１６．４ｄ，（ＣＹ）１００ｍｇ／ｋｇ，ｉｐ分别在ｄ７，ｄ１６，ｄ２６给予能显著降低小鼠外周血白细胞，经用ＰＡＰ─Ｉ后（方案同上），可显著促进其外周血白细胞数回升，荷ｓａｒｃｏｍａ１８０小鼠经ＣＹ１０ｍｇ／ｋｇ或加ＰＡＰ─Ｉ，１０ｍｇ／ｋｇ治疗１０ｄ，分别于不同时间检测外周白细胞数，结果表明与对照组相比单独用ＣＹ治疗的小鼠外周白细胞数显著降低，合用ＰＡＰ─Ｉ可显著对抗ＣＹ的降低白细胞效应，取上述小鼠骨髓细胞计数骨髓有核细胞并用［￣３Ｈ］ＴｄＲ细胞参入法检测骨髓细胞对重组鼠粒单系集落刺激因子的增殖能力，结果表明ＰＡＰ─Ｉ＋ＣＹ组的骨髓有核细胞细胞数及［￣３Ｈ］ＴｄＲ参入的ｄｐｍ值均显著高于单用ＣＹ组。小鼠于￣６０Ｃｏ照射前和／或照射后ｉｐＰＡＰ─Ｉ，１０ｍｇ／ｋｇ每周３次，结果证实ＰＡＰ─Ｉ能显著促进造血功能重建，ＰＡＰ─Ｉ给药组的脾节结数（ＣＦＵ─Ｓ＿８及ＣＦＵ─Ｓ＿１２）ｒｍＧＭ─ＣＳＦ诱导骨髓细胞增殖的ｄｐｍ值均显?

关 键 词：Phytolacca acinosa POLYSACCHARIDES CYCLOPHOSPHAMIDE Colony-forming unit in spleen(CFU- Colony stimulating factors Monocolonal antibody Polycolonal antibody 

分 类 号：R285.5[医药卫生—中药学]