Effect of Fish Oil on (-Adrenoceptors and the Activity of Adenylate Cyclase on Myocardial Membrane in Rats  

鱼油对大鼠心肌细胞膜上β－受体和腺苷酸环化酶活性的影响

作　　者：周建政[1,2] 吴葆杰[1,2] 张岫美[1,2] 

机构地区：[1]山东医科大学基础部药理学教研室 [2]北京市军事医学科学院毒物药物研究所

出　　处：《Journal of Chinese Pharmaceutical Sciences》1996年第3期154-159,共6页中国药学（英文版）

摘　　要：Effects of fish oil on β-adrenoceptors as well as the activity of adenylate cyclase (AC) on rat myocardial membrane were investigated.Supplementation with fish oil had no significant effect on basal activity of AC on myocardial membrane whereas it could markedly inhibit the AC activity stimulated by isoproterenol (ISO). Radioligand binding assays showed that supplementation with fish oil had no effect on Bmax and Kd, compared with saline control. However, supplementation with sheep oil could markedly reduce both the Kd and Bmax, compared with saline control. And the Kd of sheep oil group was greatly decreased than that of fish oil group. The results suggested that supplementation with fish oil mainly affected the activation of AC, not β-adrenoceptor itself.本文探讨了鱼油对大鼠心肌细胞膜上（－受体和腺苷酸环化酶（ＡＣ）活性的影响。鱼油１．４ｍｌ／ｋｇ每天灌胃两周对大鼠心肌细胞膜上腺苷酸环化酶（ＡＣ）的基础活性无显著影响，但可显著地抑制异丙肾上腺素（ＩＳＯ）激活的ＡＣ活性。放射配体饱和实验结果显示，同正常对照相比，鱼油的摄入对Ｂｍａｘ和Ｋｄ无显著影响，但同羊油组相比，可显著地降低β－受体与其配体的亲和力，同正常对照相比，羊油的摄入一方面可显著地升高β－受体与其配体的亲和力，另一方面又可显著地降低β－受体的数目。结果提示。

关 键 词：Fish oil EPA and DHA Adenylate cyclase Β-ADRENOCEPTOR 

分 类 号：R972.6[医药卫生—药品]