阻滞和非阻滞品系小鼠次级卵母细胞基因表达水平的比较  

Comparison of gene expression in the metaphase Ⅱ eggs between blocking and non-blocking strain mice

作　　者：吕俊杰[1,3] 逄丽丽[2] 程霄翔[1] 连秀丽[1] 初晨凤[1] 魏建恩[1] 王世鄂[1,2] 

机构地区：[1]福建医科大学基础医学院人体解剖学与组织学胚胎学系,福州350108 [2]福建医科大学基础医学院细胞与发育工程研究中心,福州350108 [3]铜陵市人民医院妇产科,铜陵244002

出　　处：《解剖学杂志》2016年第3期300-304,共5页Chinese Journal of Anatomy

基　　金：福建省科技引导性项目(2015Y0021);福建省高等学校新世纪优秀人才支持计划项目(NCETFJ-0601)

摘　　要：目的：通过基因芯片检测阻滞品系昆明（KM）小鼠和非阻滞品系B6C3F1小鼠次级卵母细胞（MⅡ卵）基因表达差异,探讨KM小鼠植入前胚体外发育阻滞是否与母源基因表达差异相关.方法：分别收集KM小鼠和B6C3F1小鼠MⅡ卵,运用基因芯片分析和real-time PCR验证,母源基因的差异表达.结果：基因芯片检测共筛出差异表达基因995个,其中B6C3F1小鼠MⅡ卵上调基因有793个;下调基因有202个.B6C3F1小鼠MⅡ卵表达上调的基因主要与调节基因转录、蛋白质合成与转运、氧化还原、生长因子等相关.结论：KM与B6C3F1小鼠植入前胚体外发育潜能不同可能与母源基因表达差异相关.Objective:To investigate the gene profiles in the metaphase Ⅱ eggs(M Ⅱ eggs) between blocking(KM) and nonblocking(B6C3Fl) stain mice and to study whether preimplantation embryos development block in vitro of KM mice is associated with the differences of the maternal gene expression.Methods:Mouse cDNA microarray was applied to analyse the gene expression profiles of KM and B6C3F1 mice MⅡ eggs,and then real-time PCR was used to validate some differently expressing genes.Results:995 genes were differently expressed between KM and B6C3F1 mice MⅡ eggs,including 793 upregulated genes and 202 down-regulated genes for B6C3F1 mice M Ⅱ oocytes.The genes up-regulated in B6C3F1 mice M Ⅱeggs were associated with gene transcription,protein biosynthesis and transportation,oxidation reduction,and growth factors.Conclusion:The differences of embryonic development potential between KM and B6C3F1 mice may be associated with the different expression of maternal genes.

关 键 词：次级卵母细胞 母源基因 基因表达 小鼠 

分 类 号：R-33[医药卫生]