miRNA-30c及其靶基因MTA-1在子宫内膜癌裸鼠皮下移植瘤中的作用  

The function of miRNA-30c and MTA-1 in xenograft of endometrial carcinoma in nude mice

作　　者：孔祥怡[1] 周怀君[1] 

机构地区：[1]南京大学医学院附属鼓楼医院妇产科,江苏南京210008

出　　处：《东南大学学报（医学版）》2016年第3期364-370,共7页Journal of Southeast University(Medical Science Edition)

基　　金：中央高校基本科研业务苗圃项目(021414340294)

摘　　要：目的:探讨过表达miRNA-30c对子宫内膜癌裸鼠皮下移植瘤生长的影响。方法:应用子宫内膜癌Ishikawa细胞系建立子宫内膜癌裸鼠皮下移植瘤模型,予裸鼠皮下移植瘤内多点注射miRNA-30c前体表达质粒,并以阴性质粒及PBS为对照。测量移植瘤体积,计算肿瘤生长抑制率;荧光定量PCR技术检测移植瘤组织miRNA-30c表达水平;蛋白质印迹技术检测移植瘤组织MTA-1蛋白表达水平;免疫组化技术检测移植瘤组织E-cadherin表达及微血管密度(MVD)。结果:成功建立子宫内膜癌裸鼠皮下移植瘤模型,miRNA-30c质粒治疗明显抑制肿瘤生长,抑制率为(46.98±5.13)%(P<0.01);上调移植瘤组织中miRNA-30c的表达(P<0.05),下调MTA-1蛋白表达(P<0.05),下调移植瘤组织MVD(P<0.05),上调移植瘤组织E-cadherin表达(P<0.05)。结论:过表达miRNA-30c可以通过下调MTA-1表达明显抑制子宫内膜癌裸鼠皮下移植瘤的生长。Objective： To investigate the inhibition effect on tumor growth by up-regulating the expression of miRNA-30 c in nude mice model. Methods： Subcutaneous xenografts of mude mice models with Ishikawa cells were established,the mice were treated with miRNA-30 c expression plasmid intratumorally,controled by negative control plasmid and PBS. Then, the xenograft volume was measured and inhibition ratio of tumor proliferation was calculated. Moreover,the expression of miRNA-30 c was analysed by Realtime-PCR and the expression of MTA-1protein was analysed by Western blot,the microvessel density and expression of E-cadherin were detected by immunohistochemistry. Results： The subcutaneous xenografts models of mude mice were established successfully.Treatment with miRNA-30 c expression plasmid inhibited the growth of xenograft and the inhibitory rate was（ 46. 98 ±5. 13） %（ P〈0. 01）. The expression of miRNA-30 c was up-regulated（ P〈0. 05） and MTA-1 protein expression was down-regulated（ P〈0. 05） respectively. Immunohistochemistry showed that the microvessel density decreased（ P〈0. 05）,the expression of E-cadherin increased（ P〈0. 05）. Conclusion： Up-regulation of miRNA-30 c can inhibit the growth of xenograft in nude mice model of endometrial carcinoma by down-regulating MTA-1.

关 键 词：miRNA-30c 子宫内膜癌 裸鼠 移植瘤 

分 类 号：R737.33[医药卫生—肿瘤] R-33[医药卫生—临床医学]