UHRF1表达下调对胃癌细胞增殖的抑制作用及机制  

Inhibitory effect of down-regulated expression of UHRF1 on cell proliferation of gastric cancer

作　　者：类维振 耿亚楠[1] 韩东升[1] 张伟[1] 周林[1] 

机构地区：[1]中国人民解放军第八十八医院,山东泰安271000

出　　处：《山东医药》2016年第21期7-9,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81402337)

摘　　要：目的探讨泛素样含PHD和环指域1(UHRF1)表达下调后对胃癌细胞增殖的抑制作用,并探讨其作用机制。方法将培养好的人胃癌细胞系(MKN45)随机分为空白对照组、阴性对照组(sh NC)和干扰组,后两组分别感染病毒sh NC、sh UHRF1,采用短发夹RNA(shRNA)技术下调MKN45细胞中UHRF1表达,XTT法测定各组细胞生长情况,平板克隆形成试验测算细胞克隆形成率,流式细胞术检测细胞周期和细胞凋亡率;裸鼠皮下成瘤试验检测细胞体内生长能力。结果 UHRF1表达下调后,MKN45细胞生长速度明显下降、克隆形成减少(P均<0.05);干扰组G0/G1期细胞比例增加,S期细胞比例减少(P均<0.05),细胞凋亡率升高(P均<0.05);裸鼠皮下肿瘤生长速度下降(P<0.01)。结论下调UHRF1的表达能够抑制胃癌细胞增殖,该作用可能通过延长细胞周期进程和诱导细胞凋亡来实现的。Objective To investigate the inhibitory effect of down-regulated expression of ubiquitin-like with PHD and ring finger domain 1（ UHRF1） on cell proliferation of gastric cancer（ GC） and to explore its mechanism. Methods The gastric cancer cell line（ MKN45） were randomly divided into the blank control group,the negative control group（ sh NC group） and the interference group（ sh UHRF1 group）; the latter two group were infected with sh NC and sh UHRF1. After UHRF1 expression was down-regulated by shRNA in MKN45 cells,the cell growth was examined by XTT and the clone formation rate was calculated by plate clone formation assay. Cell cycle and apoptosis of MKN45 cells were detected by flow cytometry（ FCM）. In vivo tumorigenicity assay was conducted to examine growth ability in vivo. Results After UHRF1 expression was down-regulated in MKN45 cells by shRNA,the cell growth significantly decreased and colony formation reduced（ all P〈0. 05）. In the sh UHRF1 group,the proportion of cells in G0/ G1 phase increased and in S phase decreased,the apoptosis rate was increased and the difference was statistically significant（ all P〈0. 05）. The tumor growth rate in nude mice was significantly decreased（ P〈0. 01）. Conclusion The down-regulation of UHRF1 expression may inhibit the proliferation of gastric cancer possibly through extending the cell cycle progression and inducing apoptosis.

关 键 词：胃肿瘤 泛素样含PHD和环指域1 细胞增殖 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]