大鼠肝癌模型和老年肝癌患者肿瘤标志物的表达分析  

Levels of serum markers of liver cancer： in a rat model of liver cancer versus elderly patients with liver cancer

作　　者：史英[1] 柳志宝[1] 郭廷廷[1] 

机构地区：[1]河北省沧州市中心医院肿瘤内科,061001

出　　处：《中华老年医学杂志》2016年第6期608-611,共4页Chinese Journal of Geriatrics

基　　金：沧州市科技计划项目（131302114）

摘　　要：目的 检测血清肿瘤标志物甲胎蛋白（AFP）、糖类抗原199（CA-199）和癌胚抗原（CEA）在大鼠肝癌模型和老年肝癌患者中的表达水平，并分析其临床意义。 方法 通过小剂量二乙基亚硝胺（DEN）腹腔注射的方法，成功建立了大鼠肝癌模型，对血清AFP、CA-199和CEA在大鼠肝癌发生过程中的表达进行比较分析；同时收集健康老年人和肝癌老年患者各80例的血液标本，对血清AFP、CA-199和CEA在健康老年人和肝癌老年患者中表达进行比较分析。 结果 与正常大鼠比较，肝癌大鼠的血清AFP、CA-199和CEA分别为（4.21±1.32）μg/L、（3.78±1.04）kU/L、（3.54±0.92）μg/L，与对照组大鼠（1.05±0.33）μg/L、（1.00±0.28）kU/L、（1.10±0.37）μg/L比较，均出现明显上调（t＝9.493、8.609、7.675，P＝0.000、0.000、0.000）。肝癌组80例老年肝癌患者的血清AFP、CA-199和CEA分别为（66.89±9.33）μg/L、（116.89±43.33）kU/L、（5. 83 ± 1. 56）μg/L，与对照组同期健康的老年体检者（2.56±1.09）μg/L、（5.56±1.26）kU/L、（1. 17 ± 0.51）μg/L比较，均出现上调（t＝14.379、17.470、10.677，P＝0.000、0.000、0.000）；肝癌组AFP、CA-199和CEA联合检测的阳性率为77.50%（62例），高于对照组1.25%（1例）（χ2＝17.260，P＝0.000）。 结论 血清AFP、CA-199和CEA联合检测有助于提高肝癌阳性检出率。Objective To detect the expression levels of serum alpha fetal protein （AFP）, carbohydrate antigen 199（CA-199） and carcinoembryonic antigen（CEA） in a rat model of liver cancer and elderly liver cancer patients, and explore its clinical significance. Methods A rat model of liver cancer was successfully established by intraperitoneal injection of a low dose of diethylnitrosamine （DEN）. The expression levels of serum AFP, CA-199 and CEA in rats were determined and compared during the development of liver cancer. Blood samples were collected from elderly subjects with normal liver and elderly liver cancer patients（n= 80, each）. The expressions of serum AFP, CA-199 and CEA were determined and compared between healthy elderly patients and elderly liver cancer patients. Results Serum levels of AFP, CA-199 and CEA were higher in liver cancer rats than in normal rats [（4.21±1.32）μg/Lvs. （1.05±0.33） μg/L,（3.78±1.04） kU/L vs. （1. 00±0. 28） kU/L, （3. 54-± 0.92） μg/L vs. （1.10±0.37） μg/L, t=9.493, 8. 609, 7. 675, respectively, all P=0. 000]. Serum AFP, CA-199 and CEA levels were higher in elderly patients with liver cancer than in elderly subjects with normal liver [（66.89±9.33）μg/L vs. （2.56±1.09） /xg/L,（116. 89±43. 33） kU/L vs. （5.56± 1.26） kU/L,（5. 83 ± 1. 56） μg/L vs. （1. 17±0.51） μg/L（t=14.379, 17.470, 10.677, respectively, all P=0. 000）. The positive rate of joint detection of AFP, CA-199 and CEA was higher in elderly patients with liver cancer than in elderly subjects with normal liver C77. 50% （62/80） vs. 1.25M（1/80）, x2 = 17. 260, P=0. 000]. Conclusions The joint detection of AFP, CA-199 and CEA can help increase the diagnostic rate of liver cancer in elderly patients.

关 键 词：肝肿瘤 生物学标记 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]