肺腺癌转移相关转录本1调控结直肠癌细胞迁移侵袭的机制  

The investigation of functions of metastasis associated lung adenocarcinoma transcript 1 in migration/invasion of colorectal cancer cells

作　　者：姚坤厚[1] 魏伦收[2] 马万里[1] 胡军红[1] 孟继明[1] 

机构地区：[1]河南大学淮河医院普通外科,开封475001 [2]河南大学淮河医院消化内科,开封475001

出　　处：《中华实验外科杂志》2016年第6期1646-1648,共3页Chinese Journal of Experimental Surgery

基　　金：河南省科技攻关计划（国际科技合作）项目（162102410092）

摘　　要：目的探讨长链非编码RNA肺腺癌转移相关转录本1（MALAT1）在肠癌中的表达与临床意义，及其促进结直肠癌细胞迁移侵袭的功能表型与分子机制。方法实时定量聚合酶链反应（Real-timePCR）检测MALAT1在配对结直肠癌组织和正常组织中的表达；RNA干扰抑制结直肠癌细胞SW620细胞中MALAT1本底表达，划痕实验和Transwell小室实验分别检测MALAT1下调后对SW620迁移和侵袭能力的影响；运用Westernblot检测上皮间质化标志物的表达。结果与正常组织比较，MALAT1在46．7％（14／30）的结直肠癌组织中的表达增高，差异有统计学意义（P〈0．05）；瞬时抑制MALAT1表达后显著抑制SW620细胞的迁移与侵袭能力；抑制MALAT1表达后，上皮间质化标志物表达显著下降。结论结直肠癌中异常高表达的MALAT1促进细胞迁移侵袭，该功能可能是通过调节上皮一间充质转化实现的。Objective To investigate the expression and clinical value of long non - coding RNA MALAT1 in colorectal cancer tissues. We also set to interpret the roles and the possible meehanismsof MALAT1 in cell migration/invasion of colorectal cancer cells. Methods Real - time quantitative polymer- ase chain reaction （Real -time PCR） was applied to examine the expression of MALAT1 in paired colorec- tal cancer tissues and normal tissues. MALAT1 was knocked down by small interfering RNA （siRNA） in SW620 cells. Wound healing and transwell assay were performed to evaluate the effects of MALAT1 on the migration and invasion abilities respectively. Western blotting was finally used to detect the expression of epithelial- mesenchymal transition （EMT） markers. Results MALAT1 was significantly increased in colorectal cancer tissues. MALAT1 was up - regulated in colorectal cancer tissue compare 46. 7% （14/30） compared with normal tissue with statistical difference （P 〈 0. 05 ）; Transient inhibition of MALAT1 in SW620 cells resulted in decreased cell migration/invasion. The expression of EMT markers were down - regulated after MALAT1 was knocked down. Conclusion Up - regulation of MALAT1 in eolorectal promotes cell migration/invasion, which might be mediated by inducing EMT.

关 键 词：肺腺癌转移相关转录本1 结直肠癌 肿瘤转移 上皮-间充质转化 

分 类 号：R735.34[医药卫生—肿瘤]