靶向沉默TPX2基因可抑制人结肠癌SW480细胞的增殖和侵袭  

TPX2 gene-silencing inhibits the proliferation and invasion of human colon cancer SW480 cells

作　　者：简捷[1] 黄缘[2] 刘利珍 李双[1] 邓峰[1] 

机构地区：[1]南昌大学第三附属医院消化内科,江西南昌330008 [2]南昌大学第二附属医院消化内科,江西南昌330006 [3]上海市嘉定区中心医院肿瘤科,上海201800

出　　处：《肿瘤》2016年第6期628-634,共7页Tumor

摘　　要：目的 :探讨靶向沉默Xklp2靶蛋白(targeting protein for Xenopus kinesinlike protein 2,TPX 2)基因对结肠癌SW480细胞增殖、侵袭的影响及其可能的作用机制。方法:将靶向沉默TPX2基因的重组载体p Magic4.1-sh RNA-TPX2转入结肠癌SW480细胞后,应用MTT法检测SW480细胞的增殖能力,Transwell小室法检测细胞的侵袭能力,RT-PCR和蛋白质印迹法检测SW480细胞中TPX2、血管内皮生长因子(vascular endothelial growth factor,VEGF)和基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)mRNA及蛋白的表达。结果 :重组载体p Magic4.1-shRNA-TPX2转染后,SW480细胞的增殖和侵袭能力均显著低于阴性对照组(阴性对照载体p Magic4.1-sh RNA-NC转染至SW480细胞)和空白对照组(未进行任何转染的SW480细胞)(P值均<0.01);p Magic4.1-shRNA-TPX2转染组SW480细胞中TPX2、VEGF、MMP-9 m RNA及蛋白的表达水平均显著低于阴性对照组和空白对照组(P值均<0.01)。结论 :靶向沉默TPX2基因能够显著抑制结肠癌SW480细胞的增殖和侵袭,这一作用可能与VEGF和MMP-9基因的表达下调有关。Objective： To investigate the effects of targeting protein for Xenopus kinesin-like protein 2 （TPX2） gene-silencing on the human colon cancer SW480 cells, and to explore its possible mechanism. Methods： After transfection with the recombination vector-targeting TPX2 gene-silencing pMagic4.1-shRNA-TPX2, the abilities of proliferation and invasion of SW480 cells were detected by MTT method and Transwell chamber assay, respectively. The expression levels of TPX2, vascular endothelial growth factor （VEGF） and matrix metalloproteinase-9 （MMP-9） mRNA and protein were measured by RT-PCR and Western blotting, respectively. Results： The abilities of proliferation and invasion of SW480 cells after transfection with the recombination vector-targeting TPX2 gene-silencing pMagic4.1-shRNA-TPX2 were lower than those of negative control （SW480 cells transfected with the pMagic4.1-shRNA-NC） and blank control cells （SW480 cells without any transfection） （all P 〈 0.01）. The expression levels of TPX2, VEGF and MMP-9 mRNA and protein of SW480 cells after transfection with pMagic4.1-shRNA-TPX2 were lower than those in the negative control and the blank control cells （all P 〈 0.01）. Conclusion： TPX2 gene-silencing can obviously inhibit the proliferation and invasion of SW480 cells. This effect maybe related to down-regulation of expressions of VEGF and MMP-9 gene.

关 键 词：结肠肿瘤 血管内皮生长因子类 基质金属蛋白酶9 细胞增殖 肿瘤浸润 

分 类 号：R735.35[医药卫生—肿瘤]