绵羊不同部位脂肪组织microRNA高通量测序及生物信息学分析  

High-throughput Sequencing and Bioinformatics Analysis on microRNAs Expressed in Adipose Tissues of Sheep

作　　者：张方[1] 胡子乔 景炅婕[1] 潘洋洋[1] 乔利英[1] 李宝钧[1] 刘建华[1] 刘文忠[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2016年第6期1093-1101,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(31372292);山西省研究生优秀创新项目(2015SY28)

摘　　要：本研究旨在挖掘绵羊不同脂肪组织中特异表达的miRNAs。构建肾周(PEF)、皮下(SUF)和尾部(TAF)脂肪组织的3个小RNA文库,利用Solexa测序技术进行测序,用Stem-loop qRT-PCR技术和生物信息学方法对测序结果进行验证和分析。结果表明,8个随机选择的miRNAs中有7个miRNAs的表达与测序结果一致。在肾周和皮下脂肪组织的sRNA文库中分别检测到128和112个保守miRNAs,分别发现198和196个新miRNAs。结合前期在尾脂中检测到miRNAs的分析结果,肾周脂肪中特异表达的有12个保守的和66个新miRNAs;皮下脂肪中特异表达的保守和新miRNAs分别为1个和30个;尾脂中特异表达的保守和新miRNAs分别为1个和34个。上述结果将为进一步研究miRNA调控绵羊的脂肪代谢提供科学依据。This study aimed to find tissue-specifically expressed miRNAs in ovine adipose tissues.Three small RNA（sRNA）libraries from perirenal,subcutaneous and tail adipose tissues were built for Solexa sequencing.The sequencing results were validated by Stem-loop qRT-PCR technology and further analyzed by bioinformatics methods.The PCR results shown that seven in eight randomly selected miRNAs were consistent with the sequencing results.In perirenal and subcutaneous adipose tissues,128 and 112conserved miRNAs were obtained,198 and 196novel miRNAs were found,respectively.Combined with the previous analysis results of miRNAs detected in tail adipose tissue,12 conserved and 66 novel miRNAs were specifically expressed in perirenal adipose tissue;one conserved and 30 novel miRNAs were specifically expressed in subcutaneous adipose tissue;and one conserved and 34 novel miRNAs were specifically expressed in tail adipose tissue.These results provide scientific bases for further research on the regulatory role of miRNA in fat metabolism.

关 键 词：绵羊 脂肪组织 MICRORNA Solexa测序 生物信息学方法 

分 类 号：S813.3[农业科学—畜牧学] S826[农业科学—畜牧兽医]