纯钛微弧氧化-壳聚糖-海藻酸钠涂层的细胞相容性研究  

Cytocompatibility of Ultrasonic Micro-arc Oxidation-sodium Alginate-chitosan Composite Coating on Pure Titanium

作　　者：李德超[1] 何梦乔[1] 李慕勤[2] 郭晓娟[2] 孟祥才[2] 

机构地区：[1]佳木斯大学口腔病防治协同创新中心,黑龙江佳木斯154007 [2]佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江佳木斯154007

出　　处：《口腔医学研究》2016年第6期559-563,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:31370979)

摘　　要：目的:通过对不同涂层表面进行成骨细胞生物相容性检测,评价其生物相容性。方法:纯钛微弧氧化组为对照A组、纯钛微弧氧化-碱处理-壳聚糖复合处理为实验B组、纯钛微弧氧化-碱处理-壳聚糖-海藻酸钠复合处理为C组。通过CCK-8实验检测不同时间细胞黏附情况以及增殖情况,激光共聚焦检测早期细胞骨架形态,扫描电镜观察试件表面形貌,ALP检测其不同时间碱性磷酸酶活性。结果:激光共聚焦显示B组和C组表面细胞的生长和黏附均优于A组,C组优于B组。CCK-8和ALP的检测结果显示3组材料表面细胞的增殖、活性的顺序为C组>B组>A组,3组的比较差异均具有统计学意义。结论:3组材料均具有良好的生物相容性,纯钛微弧氧化--碱处理-壳聚糖-海藻酸钠复合处理涂层细胞相容性最佳。Objective： To investigate the cytocompatibility of ultrasonic micro--arc oxidation--sodium alginate-- chitosan composite coating on pure titanium. Methods： The specimens in our research were divided into 3 groups： pure titanium ultrasonic micro--arc--oxidation （group A）; pure titanium ultrasonic micro--arc--oxidation chitosan group （group B）; and pure titanium ultrasonic micro--arc--oxidation--sodium alginate--chitosan group （group C）. Scanning electron microscope was used to observe the surface morphology. CCK 8 experiment was used to detect the adhesion rate and proliferation of osteoblasts. Laser confocal microscopy and scanning electron microscope were used to detect cytoskeleton microfilament stretch and Surface morphology of osteoblasts. ALP test was used to evaluate the alkaline phosphatase activity. Results： CCK 8 test showed that, with the extension of time, the OD value of group A, B, and C were increased and in the turn at C〉B〉A. CCK 8 hyperplastic experiments showed that the proliferation rate of group C and B were better than that of group A. Confocal laser microscope showed the cytoskeleton form. In a certain period of time, the spreading area and the number of microfilament and pseudopodia of group C were the best. The ALP activity was significant higher in group C and in the turn of C〉B〉A （P〈0.05）. Conclusion： All three materials showed harmonious cytocompatibility, and the best was the ultrasonic micro--arc oxidation--sodium alginate--chitosan composite coating.

关 键 词：纯钛 成骨细胞 壳聚糖 海藻酸钠 生物相容性 

分 类 号：R783.1[医药卫生—口腔医学]