鱼藤素对胆管癌TFK-1细胞诱导肿瘤新生血管生成的抑制作用  

Inhibitory effects of deguelin on cholangiocarcinoma TFK-1 cells induced angiogenesis

作　　者：张艳[1] 刘威 李军红[1] 牛静静 

机构地区：[1]郑州大学附属洛阳中心医院病理科,471000 [2]洛阳市第六人民医院 [3]西安市胸科医院病理科

出　　处：《中国实用医刊》2016年第12期31-34,共4页Chinese Journal of Practical Medicine

摘　　要：目的观察并评价鱼藤素在抑制胆管癌细胞诱导人脐静脉内皮细胞（HUVECs）增殖、迁移以及体内血管生成中的作用。方法以CCK-8检测鱼藤素对HUVECs增殖的影响；Ki-67检测鱼藤素对TFK-1细胞诱导HUVECs增殖能力的影响；Transwell细胞迁移实验以及小管形成实验检测鱼藤素对TFK-1细胞诱导HUVECs迁移和体外血管生成能力的影响；基质胶血管生成实验检测鱼藤素对TFK-1细胞在肿瘤新生血管生成能力的影响。结果鱼藤素在体外对HUVECs活性无影响；添加胆管癌TFK-1细胞培养上清24h后HUVECsKi-67表达阳性率为（97．3±0．71）％，普通1640培养基24h后HUVECsKi-67表达阳性率为（77．22±1．83）％，差异有统计学意义（P〈0．01）；添加鱼藤素1μmol／L培养24h后配-67表达阳性率为（87．86±2．81）％，与加入TFK-1上清组（97．3±0．71）％比较差异有统计学意义（P〈0．05）；将1μmol／L鱼藤素加入含有胆管癌，I’FK．1细胞上清的HU．VECs作用24h后，对照组穿膜细胞数为（236．7±13．86）个／视野，鱼藤素1μmol／L组为（94．67±7．45）个／视野，两组比较差异有统计学意义（P〈0．01）；将1μmol／L鱼藤素加入含有胆管癌TFK-1细胞上清的HUVECs作用24h后，对照组管腔样结构为（60．67±6．12）个／视野，鱼藤素1μmol／L组为（24．33±3．53）个／视野，两组比较差异有统计学意义（P〈0．01）；鱼藤素组基质胶栓瘤体中新生血管数量为（9．60±1．36）个／视野，对照组为（4．49±0．93）个／视野，两组比较差异有统计学意义（P〈0．05）；CD31和血管内皮生长因子的表达亦明显低于对照组。结论鱼藤素能够明显抑制胆管癌TFK-1细胞诱导人脐静脉内皮细胞在瘤体中新生血管生成的能力。Objective To observe and evaluate the inhibitory effects of deguelin on cholangio- carcinoma cells induced human umbilical vein endothelial cells （HUVECs） proliferation, migration and in vivo vascular generation. Methods The effects of degnelin to HUVECs on proliferation were detected by CCK-8. The effects of degnelin to cholangiocarcinoma TFK-1 cells inducing HUVECs on proliferation were detected by Ki-67. The migration and tube formation ability of HUVECs inducing by TFK-1 cells were determined by transwell assay and tube formation assay respectively. The effects of deguelin on TFK-1 cells to angiogenesis in vivo were examined by Matrigel plug assay. Results The deguelin had no effects to HUVECs on proliferation. The expression of Ki-67 of HUVECs treated by TFK-1 cells medium for 24 h was（97.3 ± 0. 71 ） % , comparing to （77.22 ± 1.83 ） % in 1640 medium, the difference was significant （P 〈 0. 01 ）, and （ 87.86 ± 2. 81 ） % in TFK-1 cells medium with addition of degnelin （ P 〈 0. 05 ）. The invaded HUVECs that treated by degnelin 1 μmol/L in TFK-1 cells medium for 24 h was （94. 67± 7.45 ）, comparing with（236.7 ± 13.86） in control group, the difference was significant（P 〈0. 01 ）. The number of new tubules that treated by degnelin 1 μmol/L in TFK-1 cells medium for 24 h was 24. 33± 3.53, comparing with 60. 67 ± 6. 12 in control group, the difference was significant （ P 〈 0. 01 ）. The number of new vessels in Matrigel plug that treated by deguelin group was 9.60 ± 1.36, comparing with 4. 49 ±0. 93 in control group, the difference was signiflcant（P 〈0. 05）, and the expression of CD31 and VEGF was inhibited by deguelin. Conclusions Degnelin could inhibit the angiogenesis of human um- bilical vein endothelial cells that induced by cholangiocarcinoma TFK-1 cells.

关 键 词：鱼藤素 胆管肿瘤 血管生成 细胞迁移 

分 类 号：R735.8[医药卫生—肿瘤]