Structural and metabolic responses of microbial community to sewage-borne chlorpyrifos in constructed wetlands  

作　　者：Dan Zhang Chuan Wang Liping Zhang Dong Xu Biyun Liu Qiaohong Zhou Zhenbin Wu 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Journal of Environmental Sciences》2016年第6期4-12,共9页环境科学学报（英文版）

基　　金：supported by the Major Science and Technology Program for Water Pollution Control and Treatment of China 12th Five-Year Plan (No. 2012ZX07101007-005);the National Natural Science Foundation of China (Nos. 30870221, 51178452, 50909091);the Foundation for Innovative Research Groups of the Natural Science Foundation of Hubei Province,China (2010CDA066);the National Science Foundation for Distinguished Young Scholars of Hubei Province, China (No. 2010CDA093)

摘　　要：Long-term use of chlorpyrifos poses a potential threat to the environment that cannot be ignored, yet little is known about the succession of substrate microbial communities in constructed wetlands（CWs） under chlorpyrifos stress. Six pilot-scale CW systems receiving artificial wastewater containing 1 mg/L chlorpyrifos were established to investigate the effects of chlorpyrifos and wetland vegetation on the microbial metabolism pattern of carbon sources and community structure, using BIOLOG and denaturing gradient gel electrophoresis（DGGE） approaches. Based on our samples, BIOLOG showed that Shannon diversity（HV） and richness（S） values distinctly increased after 30 days when chlorpyrifos was added. At the same time, differences between the vegetated and the non-vegetated systems disappeared. DGGE profiles indicated that H Vand S had no significant differences among four different treatments. The effect of chlorpyrifos on the microbial community was mainly reflected at the physiological level. Principal component analysis（PCA） of both BIOLOG and DGGE showed that added chlorpyrifos made a difference on test results.Meanwhile, there was no difference between the vegetation and no-vegetation treatments after addition of chlorpyrifos at the physiological level. Moreover, the vegetation had no significant effect on the microbial community at the genetic level. Comparisons were made between bacteria in this experiment and other known chlorpyrifos-degrading bacteria. The potential chlorpyrifos-degrading ability of bacteria in situ may be considerable.Long-term use of chlorpyrifos poses a potential threat to the environment that cannot be ignored, yet little is known about the succession of substrate microbial communities in constructed wetlands（CWs） under chlorpyrifos stress. Six pilot-scale CW systems receiving artificial wastewater containing 1 mg/L chlorpyrifos were established to investigate the effects of chlorpyrifos and wetland vegetation on the microbial metabolism pattern of carbon sources and community structure, using BIOLOG and denaturing gradient gel electrophoresis（DGGE） approaches. Based on our samples, BIOLOG showed that Shannon diversity（HV） and richness（S） values distinctly increased after 30 days when chlorpyrifos was added. At the same time, differences between the vegetated and the non-vegetated systems disappeared. DGGE profiles indicated that H Vand S had no significant differences among four different treatments. The effect of chlorpyrifos on the microbial community was mainly reflected at the physiological level. Principal component analysis（PCA） of both BIOLOG and DGGE showed that added chlorpyrifos made a difference on test results.Meanwhile, there was no difference between the vegetation and no-vegetation treatments after addition of chlorpyrifos at the physiological level. Moreover, the vegetation had no significant effect on the microbial community at the genetic level. Comparisons were made between bacteria in this experiment and other known chlorpyrifos-degrading bacteria. The potential chlorpyrifos-degrading ability of bacteria in situ may be considerable.

关 键 词：Constructed wetland Chlorpyrifos BIOLOG DGGE 

分 类 号：X172[环境科学与工程—环境科学] X703