基于自由基反应荧光增强的葡萄糖检测  

Determination of glucose by turn-on fluorescence method based on radical reactions

作　　者：牛静[1] 胡鹏[1] 赵梦溪[1] 尹海燕[2] 王红旗[2] 刘继红[2] 

机构地区：[1]黄淮学院化学化工系,驻马店463000 [2]河南省农业科学院农业质量标准与检测技术研究所,郑州450002

出　　处：《食品安全质量检测学报》2016年第5期1934-1938,共5页Journal of Food Safety and Quality

基　　金：国家农产品质量安全风险评估项目(2016);河南省高等学校重点项目(16A150015);河南省农业科学院科研发展专项资金项目(20157813)~~

摘　　要：目的建立一种基于自由基反应荧光增强的葡萄糖检测方法。方法葡萄糖分子被葡萄糖氧化酶氧化,酶反应产物H_2O_2和Fe^(3+)一起组成Fenton试剂,Fe^(3+)催化H_2O_2产生羟基自由基,催化产物迅速的和OPDA反应生成一种荧光低聚物并产生增强的荧光信号,实现葡萄糖的间接检测。结果在0.20~12.50 mmol/L浓度范围内,荧光强度和葡萄糖浓度呈线性关系,相关系数r=0.98,检测限为25μmol/L。结论该方法具有较高的灵敏度和简便的操作步骤,并且易于实现定量分析。Objective To construct a fluorescence sensing platform for the determination of glucose based on the free radical reactions.Methods H_2O_2 which was the oxidative product of glucose and its oxidase were catalyzed by Fe^3＋.The catalytic reaction product could quickly react with o-phenylenediamine,and generate a fluorescent oligomer and an enhanced signal of fluorescence.Thereby the detection of glucose was indirectly realized.Results The fluorescent intensity was linearly dependent on the glucose concentration in the range of 0.20~2.50 mmol/L with the correlation coefficient of 0.98,and the detection limit was 25 μmol/L.Conclusion The method is sensitive and simple,which can be applied for quantitative analysis.

关 键 词：葡萄糖 自由基反应 荧光 

分 类 号：O657.3[理学—分析化学] R446.1[理学—化学]