树舌灵芝总三萜提取工艺优化及其抗氧化作用研究  

Extraction process optimization and its antioxidant activity of the total triterpenoids from Ganoderma applanatum

作　　者：贾瑞博[1,2] 赵慧[1,2] 刘凯丽[1,2] 李燕[1,2] 周文斌[1,2] 吕旭聪[1,2] 刘斌[1,2] 

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]国家菌草工程技术研究中心,福建福州350002

出　　处：《应用化工》2016年第6期1030-1035,共6页Applied Chemical Industry

基　　金：国家科技支撑计划子课题(2014BAD15B01);福建省科技重大专项(2014NZ2002-1);中国博士后科学基金项目(2015M570549);福建省教育厅科技计划项目A类课题(JA14107)

摘　　要：以树舌灵芝为原料,研究总三萜的提取工艺及其抗氧化性。选择料液比、乙醇浓度、超声温度和超声时间进行单因素实验,在单因素实验基础上,采用Box-Behnken试验设计,以树舌灵芝总三萜得率为响应值进行响应面分析,确定最优工艺参数为:料液比1∶20(g/m L),乙醇浓度70%,超声温度55℃,超声时间60 min。在此工艺条件下进行5次验证性实验,树舌灵芝总三萜的提取得率平均值为1.75%。并通过测定DPPH的清除率、羟基自由基的清除率以及总还原力,研究其抗氧化活性。The objective of the study is optimum ultrasonic-assisted extraction process and inquiry antioxidant activity of total triterpenoids from Ganoderma applanautm（ Pers.）.Carrying out single factor experiments by choosing solid-liquid ratio,ethanol concentration,temperature and extraction time.Based on the results of single factor experiment,using Box-Behnken experimental design with four factors and three levels.The optimal extraction technology of triterpenoids was as follows： G.applanatum were extracted with70% ethanol at the solid-liquid ratio of 1 ∶20（g/mL） for 60 min at 55 ℃.Under these conditions,the average extraction rate of triterpenoids was 1.75%.Study its antioxidant activity by measuring the clearance of DPPH,the clearance of ·OH and the total reducing power.

关 键 词：树舌灵芝 总三萜 响应面 工艺优化 抗氧化 

分 类 号：TQ914.1[化学工程]