硫化氢对人卵巢癌细胞SKOV3细胞增殖的影响  

Effects of Hydrogen Sulfideon the Proliferation of Thehuman Ovarian Carcinoma Cells SKOV3

作　　者：吕锡芳[1] 陈志刚[2] 何雄伟[3] 

机构地区：[1]石河子大学医学院第一附属医院妇产科,新疆石河子市832000 [2]石河子大学医学院第一附属医院中心实验室,新疆石河子市832000 [3]石河子绿洲医院

出　　处：《医学理论与实践》2016年第10期1276-1278,共3页The Journal of Medical Theory and Practice

基　　金：新疆生产建设兵团医药专项课题(项目编号:2013GS650104)

摘　　要：目的:探讨不同浓度NaHS(硫化氢供体)对人卵巢癌SKOV3细胞增殖的影响。方法:体外培养人卵巢癌SKOV3细胞,分为正常对照组、NaHS(25μmol/L)组、NaHS(50μmol/L)组、NaHS(75μmol/L)组、NaHS(100μmol/L)组,采用MTT法检测各组干预48h后SKOV3细胞增殖情况,流式细胞术检测各组干预48h后SKOV3细胞周期分布情况。结果:与正常对照组比较,NaHS(25μmol/L)组、NaHS(50μmol/L)组、NaHS(75μmol/L)组、NaHS(100μmol/L)组SKOV3细胞抑制率均显著增加,G0/G1期细胞均显著增加,S期细胞均显著减少,细胞增殖指数均明显降低;与NaHS(25μmol/L)组比较,NaHS(50μmol/L)组、NaHS(75μmol/L)组、NaHS(100μmol/L)组SKOV3细胞抑制率均显著增加,G0/G1期细胞均显著增加,S期细胞均显著减少,细胞增殖指数均明显降低;与NaHS(50μmol/L)组比较,NaHS(75μmol/L)组、NaHS(100μmol/L)组SKOV3细胞抑制率均明显降低,G0/G1期细胞均明显减少,S期细胞均明显增加,细胞增殖指数均明显降低;而NaHS(75μmol/L)组在细胞抑制率、G0/G1期细胞比例、S期细胞比例、细胞增殖指数等方面与NaHS(100μmol/L)组无明显差异。结论:硫化氢可抑制人卵巢癌SKOV3细胞的增殖,并呈明显浓度依赖性。Objective：To investigate the effects of hydrogen sulfide on the proliferation of the human ovarian carcinoma cells SKOV3.Methods：The cultured human ovarian carcinoma cells SKOV3 were divided into the normal control group,hydrogen sulfide（25μmol/L）group,hydrogen sulfide（50μmol/L）group,hydrogen sulfide（75μmol/L）group and the hydrogen sulfide（100μmol/L）group,the cultured human ovarian carcinoma cells were treated for 48 hours.The cells proliferation was measured by MTT assay.The cells cycle was measured by flow cytometry.Results：Compared with the normal control group,the Cell inhibition rate in the hydrogen sulfide（25μmol/L）group,hydrogen sulfide（50μmol/L）group,hydrogen sulfide（75μmol/L）group and the hydrogen sulfide（100μmol/L）group were increased.All the cells of G1 phase were significantly increased and the cells of S phase were significantly decreased.Compared with the hydrogen sulfide（25μmol/L）group,the Cell inhibition rate in the hydrogen sulfide（50μmol/L）group,hydrogen sulfide（75μmol/L）group and the hydrogen sulfide（100μmol/L）group were significantly increased.All the cells of G1 phase were significantly increased and the cells of Sphase were significantly decreased.Compared with the hydrogen sulfide（50μmol/L）group,the cell inhibition rate in the hydrogen sulfide（75μmol/L）group and hydrogen sulfide（100μmol/L）group were significantly increased.All the cells of G1 phase were significantly increased and the cells of S phase were significantly decreased.Conclusion：The hydrogen sulfide can inhibit the human ovarian carcinoma cells proliferation in aconcentration-dependent manner.

关 键 词：卵巢癌细胞 硫化氢 细胞增殖 流式细胞术 

分 类 号：R737.3[医药卫生—肿瘤]