拮抗内生细菌Bacillus mojavensis ZA1在马铃薯根内及根际的定殖动态及其对土壤微生物的影响  

Colonization of Antagonistic Endophytic Bacteria Bacillus mojavensis ZA1 in the Root of Potato and Rhizosphere Soil and Its Effect on Soil Microorganism

作　　者：王颖[1] 杨成德[1] 薛莉[1] 崔月贞 杨小利[1] 

机构地区：[1]甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,兰州730070

出　　处：《中国生物防治学报》2016年第3期372-378,共7页Chinese Journal of Biological Control

基　　金："十二五"国家科技支撑计划(2012BAD06B03);国家自然科学基金(31160122)

摘　　要：利用GFP标记和平板分离法研究了拮抗内生细菌Bacillus mojavensis ZA1在马铃薯根内及根际的定殖动态及其对土著微生物的影响。结果表明,标记菌株ZA1-gfp在土壤中定殖数量为3.7×10^4～7.3×10^4 cfu/g;马铃薯根际为3.0×10～3～1.5×10^5 cfu/g,根内为49～630 cfu/g;马铃薯的根部横切面、根毛和根表皮等部位均观察到发绿色荧光的菌株ZA1-gfp菌体或聚集菌落。利用灌根法施入生防菌后,土壤中细菌、放射菌数量显著增加（P〈0.05）,土壤真菌数量在中期下降;土壤中细菌、真菌和放线菌的数量分别在相对稳定范围内（数据之间差异不显著）消长。该结果说明菌株ZA1-gfp能够在马铃薯根内及根际定殖且不影响土壤中土著微生物的群落稳定性。GFP labelling technique and plate culture method were used to study the colonization of endophytic bacterium Bacillus mojavensis ZA1 in the root of potato and rhizosphere soil and its effect on soil microorganism. The results showed that ZAl-gfp colonized well in soil with a density of 3.7 ×104 to 7.3 × 104 cfu/g and colonized in the roots of potato and rhizosphere with densities of 49 to 630 cfu/g and 3.0× 103 to 1.5 × 105 cfu/g, respectively. ZA l-gfp could be detected in inner roots and on the root hairs and root epidermis of potato under a fluorescence microscope. The number of soil bacteria and actinomycetes were increased significantly （P~ 0.05） after ZAl-gfp inoculation using root-drenching method, but fungi was decreased in 25 days later after ZAl-gfp inoculation; the number of soil bacteria, fungi and actinomycetes was kept well （There was no obvious difference）. The results showed that ZA 1-gfp could steadily colonize the roots of potato and rhizosphere and the community stability of soil microorganisms was kept well.

关 键 词：拮抗内生细菌 GFP标记 马铃薯 定殖动态 土壤微生物 

分 类 号：S476[农业科学—农业昆虫与害虫防治]