脑内注射染锰对大鼠行为学及黑质多巴胺能神经元的影响  

Influence of manganese exposure via intracerebral injection on behavioristics and substantia nigra dopaminergic neurons in rats

作　　者：莫瑞康 王进[1] 黄坚毅 文巧林 黄锦凤[1] 

机构地区：[1]广西医科大学第一附属医院神经内科,南宁530021 [2]广西崇左市人民医院神经内科

出　　处：《中华劳动卫生职业病杂志》2016年第6期406-411,共6页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目（81460181）;广西科学研究与技术开发计划项目资助项目（09-090-05）;广西自然科学基金资助项目（桂科自0728147）

摘　　要：目的探讨经脑内注射染锰后大鼠行为学、黑质酪氨酸羟化酶（TH）免疫阳性细胞和黑质神经元超微结构的变化。方法将72只健康雄性SD大鼠随机分为染毒组和对照组，每组36只。采用立体定向技术向染毒组大鼠纹状体内注射1μl MnCl2·4H2O（1mol／L），对照组注射等量体积的生理盐水，于注射后各时间点（8h和1、3、7、15、30d，每个时间点6只）观察阿朴吗啡诱发的大鼠旋转行为、黑质TH免疫阳性细胞数以及黑质超微结构变化，并与腹腔注射染锰方法进行比较。结果阿朴吗啡诱导30min后，染毒组大鼠的旋转圈数随染锰时间延长呈逐渐增加趋势（F=176．921，P〈0．01），染毒组大鼠的旋转圈数明显高于对照组，差异亦有统计学意义（F=482．654，P〈0．01）。染毒组大鼠损伤侧黑质的TH免疫阳性细胞平均吸光度（A）值随时间延长呈逐渐下降趋势（F=12．009，P〈0．01），与同组对侧及对照组损伤侧相比，差异有统计学意义（F=36．131，P〈0．01）；染毒组大鼠第3、7、15、30天损伤侧黑质TH免疫阳性细胞平均A值明显低于同组对侧，差异均有统计学意义（均P〈0．01）；第7、15、30天，其平均A值与对照组损伤侧相比明显降低，差异均有统计学意义（均P〈0．01）。大鼠黑质神经元染锰后不同阶段呈现出线粒体肿胀、内质网扩张、神经纤维脱髓鞘等改变，提示多巴胺能神经元损伤的动态过程。与腹腔注射染锰比较，脑内注射染锰诱发大鼠出现行为学改变和黑质-纹状体系统损伤的时间较短。结论脑内注射染锰方法可较短时间内造成大鼠行为学改变以及黑质多巴胺能神经元损伤。Objective To investigate the changes in behavioristics, substantia nigra tyrosine hydroxylase （TH） immunoreactive cells, and uhrastructure of substantia nigra neurons in rats after manganese exposure via intracerebral injection. Methods A total of 72 healthy male Sprague-Dawley rats were randomly divided into exposure group and control group, with 36 rats in each group. The stereotactic technique was used for injection of 1 μl MnCl2-dH20（ 1 mol/L） into the corpus striatum in the exposure group, and the control group was injected with the same volume of normal saline. The changes in rotational behavior, number of TH immunoreactive cells in the substantia nigra, and uhrastructure of the substantia nigra induced by apomorphine were observed at 8 hours and 1, 3, 7, 15, and 30 days after injection, and this measure was compared with manganese exposure via intraperitoneal injection. Results After apomorphine induction for 30 minutes, the exposure group showed a gradual increase in the number of rotations over the time of manganese exposure （F= 176.921, P〈0.01 ） and a significantly higher number of rotations than the control group （F=482.654, P〈0.01 ）. The exposure group showed a gradual reduction in the mean A value of TH immunoreactive ceils in the injured substantia nigra over the time of exposure （F= 12.009, P〈0.01 ）, and this value differed significantly between the injured substantia nigra in the exposure group and the contralateral substantia nigra in the exposure group and the injured side in the control group（F=36.131, P〈0.01 ）. At 3, 7, 15, and 30 days after exposure, the injured substantia nigra showed a significantly lower mean A value of TH immunoreactive cells than the contralateral side in the exposure group （all P〈0.01 ）. At 7, 15, and 30 days, the injured substantia nigra in the exposure group showed a significantly lower mean A value than the injured side in the control group （all P〈0.01 ）. Aftermanganese exposure, substantia nigra neurons showed the changes

关 键 词：立体定位技术 锰 多巴胺能神经元 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]