基于转基因小鼠构建病证结合脾虚模型的探讨  

Constructing Integrative Disease and Syndrome Model of Spleen-deficiency with Transgenic Mice

作　　者：孙学刚[1] 靖林林[2] 

机构地区：[1]南方医科大学中医药学院,广东广州510515 [2]南方医科大学中西医结合医院,广东广州510305

出　　处：《辽宁中医杂志》2016年第6期1329-1331,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81273621;81573848);广东省自然科学基金(2014A030313323)

摘　　要：目前以苦寒泻下、饥饱劳倦等方法诱导的脾虚动物模型存在规范性、特异性等方面问题。基因突变小鼠如APC^(min/+)、基因敲除小鼠Txnip^(-/-)、IP3R2^(-/-)、db/db小鼠以及条件性敲除Pten小鼠具有易感肿瘤、肌肉失养、肥胖、能量代谢障碍等特征,与脾虚运化失司、清阳不升、聚湿生痰和抗病能力降低相关。基于转基因小鼠结合饥饱劳倦等方法构建的病证结合脾虚模型更为客观、可信和可重复性,该类模型具有较好的转化潜力并将有助于深化脾虚证侯的本质研究。Normative,specificity and other problems exist in spleen deficiency models caused by bitter- cold diarrhea or hunger- satiety methods. Transgenic mice,such as APCmin/＋mice,Txnip-/-mice,Pten＋/-mice,IP3R2-/-mice and db/db mice,are characterized with tumor microenvironment,muscle dystrophy,obesity,metabolic disorders and have relations with spleen deficiency and dysfunction,clear Qi not ascending,phlegm due to damp accumulation and susceptible to disease. Therefore,construction of spleen- deficiency models with transgenic mice would be objective and credible. The high reproducibility of these models has translation potential and would accelerate the research essence of combination of disease and syndrome.

关 键 词：脾虚证 动物模型 转基因小鼠 病证结合 

分 类 号：R-332[医药卫生]