藤黄酸对人结直肠癌细胞凋亡及Bax、Bcl-2和Caspase-3表达的影响  

Effect of gambogic acid on cell apoptosis and expressions of Bax,Bcl-2 and Caspase-3 in colorectal cancer cells with

作　　者：魏建昌[1] 张通[1] 杨平[1] 曾山崎[1] 王成兴[1] 陈华翠[1] 曹杰[1] 

机构地区：[1]广州医科大学附属广州市第一人民医院胃肠外科,广州消化疾病中心,510180

出　　处：《实用医学杂志》2016年第11期1745-1748,共4页The Journal of Practical Medicine

基　　金：国家自然科学资金项目(编号:81272556)

摘　　要：目的:研究藤黄酸对结直肠癌细胞凋亡及Bax、Bcl-2和Caspase-3表达的影响。方法:藤黄酸对SW480、LOVO细胞进行干预,CCK-8法检测增殖抑制;光镜观察细胞形态变化;免疫荧光染色技术检测凋亡;Western Blot法测定Bax、Bcl-2、Caspase-3蛋白的表达。结果:藤黄酸抑制SW480、LOVO细胞增殖,且抑制作用随浓度及时间的增加而增强(P<0.05);藤黄酸诱导SW480、LOVO细胞发生凋亡,增强Bax、Caspase-3蛋白的表达,减弱Bcl-2蛋白的表达,提高Bax/Bcl-2比值(P<0.05)。结论:藤黄酸抑制SW480、LOVO细胞增殖并诱导凋亡,其机制可能与提高Bax/Bcl-2比值、激活Caspase-3相关。Objective To investiagate cell apoptosis and expressions of Bax,Bcl-2 andCaspase-3 in gambogic acid-treated eolorectal cancer cells. Methods SW480/LOVO coloreetal cancer cells were treated by gambogic acid. Cell Counting Kit-8 assay （CCK-8） was used to test cell proliferation. Microscopy was used to check the morphological changes. Immunofluorescence staining technique was used to detect cell apoptosis. Expressions of Bax,Bcl-2 and Caspase-3 protein were detected by Western blot assay. Results Gambogic acid inhibited the proliferation of SW480/LOVO in a dose and time-dependent manner. Gambogic acid could induce cell apoptosis. Gambogic acid increased expressions of Caspase-3 and Bax, increased the ratio of Bax/Bcl-2, and decreased Bcl-2 protein expression. Conclusion Gambogic acid can inhibit proliferation and induce apoptosis of SW480LOVO cells, with the mechanism of up-regulation of Bax/Bcl-2 and activation of Caspase-3.

关 键 词：结直肠肿瘤 藤黄酸 凋亡 BAX/BCL-2 CASPASE-3 

分 类 号：R735.34[医药卫生—肿瘤]