快速定量检测CA72-4的超顺磁性免疫层析试纸条的研制  

Development of an superparamagnetic immunochromatographic test strip for rapid quantification of CA72-4

作　　者：陈艳荣[1] 王侃[2] 刘宗瑞[1] 卢文婷[1] 何井华[1] 

机构地区：[1]南方医科大学珠江医院,广州市510280 [2]上海交通大学电子信息与电气工程学院仪器科学与工程系,上海智能诊疗仪器工程技术研究中心,上海市200000

出　　处：《实用医学杂志》2016年第11期1753-1756,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:81571835)

摘　　要：目的:建立一种简单、实用的磁性免疫层析试纸条来检测人血清中糖链抗原72-4(CA72-4)水平。方法:采用水热合成法制备羧基化水溶性纳米磁珠,利用EDC/NHS偶联技术制备纳米磁珠探针,优化并组装试纸条,对其灵敏度、特异度及稳定性进行测试及评价。结果:构建的试纸条不仅能提供定性的检测结果,还可以通过稳定的磁信号进行高灵敏度的定量分析,定量检测限值为0.83 IU/m L。检测100例临床血清样本,结果与电化学发光法一致性较高,其灵敏度为99%,特异度为93%,差异具有统计学意义。试纸条及磁信号强度稳定性良好。结论:初步实现了利用免疫层析试纸条快速定量检测CA72-4,且检测灵敏度高、特异性好,试纸条及磁信号强度稳定性良好。检测过程简单、快速,适合大样本的初筛或者作为胃癌患者进行病情监测的自检测手段。Objective To establish a simple and practical superparamagnetic immunochromatographic test strip for rapidly monitoring human serum level of CA72-4. Methods Water-soluble carboxylated superparamagnetic nanoparticles were prepared with a modified one-step hydrothermal synthesis method. Magnetic probe was prepared by immobilizing specific antibody （mAbl） onto the surface of nanoparticles. Following with optimization and assembly of the test strip, we evaluated sensitivity, specificity, stability of this method for serum CA72-4 detection. Results The optimized test strip provided not only the qualitative results, but also the high sensitivity quantitative detection through stable magnetic signal. The detection limit was 0.83 IU/mL. One hundred clinical samples （70 positive and 30 negative） were measured to assess these test strips with high sensitivity （99%） and high specificity （93%）. The test strip and magnetic signal possessed high stability. Conclusion A rapid and quantitative detection of CA72-4 by the test strip was accomplished. This method is rapid, sensitive and quantitative, possessing great potential in large sample screening or in-home testing.

关 键 词：糖链抗原72—4(CA72—4) 超顺磁性纳米磁珠 免疫层析法 快速定量检测 

分 类 号：R730.43[医药卫生—肿瘤]