急性高台应激对大鼠神经内分泌激素、相关受体及脑神经递质的影响  

The impact of exposure to acute elevated platform on the expression of neuroendocrine hormones, re- ceptors, and neurotransmitters of rats

作　　者：韦姗姗[1] 杨和金 黄加文 陆雪萍 彭玲芳 王庆国[1] 

机构地区：[1]北京中医药大学基础医学院,100029 [2]云南省药物研究所药物筛选中心,昆明650111

出　　处：《国际免疫学杂志》2016年第3期205-209,共5页International Journal of Immunology

基　　金：国家重点基础研究发展计划（973计划）（2011CB505100）

摘　　要：目的高台应激是一种不可逃避应激，是研究应激对机体神经生理病理变化的重要模型。本研究对急性高台应激后神经内分泌激素、受体表达、脑神经递质变化以及地西泮的干预作用进行探讨。方法大鼠随机分为空白对照组、应激＋地西泮（DAP）组与应激＋溶剂组。后两组于应激前30min分别腹腔注射地西泮2mg／kg与等量生理盐水。采用酶联免疫法测量应激后各组的血浆促。肾上腺皮质激素（ACTH）、血清皮质酮（CORT）水平；采用实时定量PCR测量下丘脑促肾上腺皮质激素分泌激素（CRH）mRNA、海马糖皮质激素受体（GR）mRNA、盐皮质激素受体（MR）mRNA、5-羟色胺1a受体（5-HT1aR）mRNA水平；采用高效液相色谱电化学法测量大脑皮层匀浆液中去甲肾上腺素（NE）、多巴胺（DA）、5-羟色胺（5-HT）及其代谢产物5一羟吲哚乙酸（5-HIAA）水平。结果与空白组相比，应激＋溶剂组大鼠血浆ACTH、血清CORT以及海马5-HT1aRmRNA水平升高（P均〈0．05），此变化可由DAP逆转（P均〈0．05）。此外，DAP还可降低应激后的下丘脑CRHmRNA，海马GRmRNA以及MRmRNA水平（P均〈0．05）。然而大脑皮层匀浆液中NE、DA、5-HT、5-HIAA在应激后无变化。结论急性高台应激可引起大鼠相关神经内分泌激素与受体表达变化，且该效应可被DAP逆转。Objects Elevated platform stress is an important model to study the acute stress-induced neurophysiologic changes of organisms. In this study, we investigated the effect of the elevated platform stress and/or diazepam （DAP） on the expression of neuroendocrine hormones, receptors, and neurotransmitters in rats. Methods Rats were randomly divided into three groups： naive group, stress ＋ DAP group, and stress ＋ vehicle group. The last two groups were given DAP （ 2 mg/kg in vehicle, i. p. ） and vehicle （ i. p. ） 30 min a- head of stress, respectively. The levels of plasma adrenocorticotropie hormone （ACTH） and serum eorticoster- one （CORT） were determined by enzyme-linked immuno sorbent assay （ELISA）. The mRNA level of hypo- thalamus eorticotropin releasing hormone （ CRH）, hippocampus glueoeorticoid receptors （ GR）, mineralocorti- coid receptor （MR）, and 5-hydroxytryptophan receptor （5-HT1 aR） were determined by quantitative real-time PCR. The amount of norepinephrine （NE）, dopamine （DA）, 5-hydroxytryptophan （5-HT）, and 5-hydroxyin- doleacetic acid （5-HIAA） were determined by HPLC-Electrochemical Detection. Results Compared with the naive group, levels of plasma ACTH, serum CORT, and hippocampus 5-HT1 aR mRNA in the last two groups increased after exposure to the stress （ all p 〈 0.05 ）. Such increases could be reversed by DAP （ all P 〈 0. 05 ）. In additional, DAP could down-regulate the mRNA level of hypothalamus CRH, hippocampus GR and MR （ all P 〈 0.05）. Meanwhile, elevated stress and DAP have no significant effect on the NE, DA, 5-HT, and 5-HIAA level. Conclusion The acute elevated platform stress induces higher levels of neuroendoerine hormones and re- ceptors in rats. This effect can be reversed by DAP.

关 键 词：高台应激 神经内分泌激素 受体 神经递质 

分 类 号：R363[医药卫生—病理学]