救必应水提取物对产ESBLs细菌的抑菌机理研究  

Study on the Bacteriostatic Mechanism of the Water Extracts of Holly Bark on ESBLs-producing Bacterial

作　　者：宋剑武[1] 吴永继 孙燕杰[1] 刘增援 赵雨川 胡梅[1] 夏娟[1] 黄凯[1] 司红彬[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《中国畜牧兽医》2016年第6期1536-1543,共8页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31460675);国家科技支撑计划(2012BAD25B04);广西科技攻关项目(0992014-2);广西自然基金面上基金项目(2013GXNSFAA019070);南宁市科技攻关项目(20141295);广西高校优势特色专业建设项目

摘　　要：为了探讨救必应水提取物的抑菌作用及抑菌机理,采用2倍微量稀释法和琼脂平板稀释培养计数法,测定救必应水提取物对产ESBLs(extended spectrumβ-lactamases)细菌的最小抑菌浓度(minimal inhibitory concentration,MIC)和最小杀菌浓度(minimal bactericidal concentration,MBC),通过对细菌生长曲线、细菌超微结构、细胞壁和细胞膜通透性变化试验、核酸合成抑制试验研究救必应水提取物的抑菌机理。结果表明,与不添加提取物的对照组相比,救必应水提取物能够影响细菌的生长规律,使细菌破损严重,细胞壁和细胞膜通透性增加,细胞质外渗,菌液中AKP含量、可溶性蛋白含量增多、大肠杆菌DNA荧光强度明显减弱。研究表明,救必应水提取物的抑菌机理是通过细菌细胞壁和细胞膜通透性的变化,抑制细菌核酸的合成实现的。The study was undertaken to study the inhibitory effect and mechanism of the water extracts of Holly Bark.Minimal inhibitory concentration（MIC）was tested through twice micro-dilution method and minimal bactericidal concentration（MBC）was tested by agar plate dilution counting method（SDA）of the water extracts of Holly Bark on producing extended spectrumβ-lactamases（ESBLs）bacteria,then the bacteriostatic mechanism of the water extracts of HollyBarkthrough bacteria curve was tested,the cell ultrastrueture was observed,permeabilty changes of cell wall and membrane,nucleic acid synthesis inhibitory experiment were researched.The results showed that the water extracts of Holly Bark could affect bacteria curve compared with the blank control,destroy the cells seriously,permeability of both cell membrane and wall increased,extravasation of cytoplasm appeared,the content of AKP and soluble protein in the bacterial liquid increased,E.coli DNA fluorescence intensity weakened obviously.Research showed that the bacteriostatic mechanism of the water extracts of HollyBark was based on its roles about changes on the cell wall and membrance permeability,inhibitting nucleic synthesis of bacterial.

关 键 词：救必应 产ESBLS细菌 最小抑菌浓度 最小杀菌浓度 抑菌机理 

分 类 号：S855.1[农业科学—临床兽医学]