Identification of Gardenoside and Sarsasapogenin from Qingwen Baidu Granules by TLC  

作　　者：Guo Shijin Wang Yanping Zhang Zhimei Fu shijun Xu Qianqian Zhang Ying Shen Zhiqiang 

机构地区：[1]Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China [2]Shandong Lvdu Ante Veterinary Drug Co. , Ltd. ,Binzhou 256600, China

出　　处：《Animal Husbandry and Feed Science》2016年第2期79-81,共3页动物与饲料科学（英文版）

基　　金：Supported by Technical Innovation Project of Shandong Province(201210916001)

摘　　要：[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone（9：l ） as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water （10：7：2：0.5） as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone（9：l ） as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.

关 键 词：Qingwen baidu granules Gardenoside SARSASAPOGENIN TLC identification 

分 类 号：S853.7[农业科学—临床兽医学]