氯化钴对大鼠脑缺血模型基质细胞衍生因子-1及趋化因子受体4表达的影响  

Effects of cobalt chloride on the expressions of stromal cell derived factor 1 and chemokine receptor 4 in cerebral ischemia rats

作　　者：李佳[1] 邹常林[1] 张志明[1] 张春阳[2] 

机构地区：[1]天津中医药大学附属南开医院,天津300100 [2]河北联合大学附属医院神经内科,河北唐山063000

出　　处：《中国临床药理学杂志》2016年第12期1109-1111,共3页The Chinese Journal of Clinical Pharmacology

基　　金：河北省医学科学研究课题计划基金资助项目(ZL20140185)

摘　　要：目的研究氯化钴预处理对脑缺血大鼠基质细胞衍生因子(SDF-1)、CXC家族趋化因子受体4(CXCR4)蛋白表达的影响。方法 45只SD大鼠,随机分为假手术组、模型组、实验组,每组均15只。模型组及实验组,用线栓法制作脑缺血模型;在造模前2天与前1天,实验组腹腔注射氯化钴30 mg·kg^(-1)。用蛋白质印迹法检测各组大鼠脑组织SDF-1、CXCR4蛋白表达,原位末端标记法(TUNEL)检测各组大鼠脑细胞凋亡率,用氯化三苯基四氮唑(TTC)染色并计算2 d后脑组织缺血坏死面积。结果在各时间点,与假手术组(脑组织SDF-1、CXCR4蛋白表达极低)比较,实验组与模型组SDF-1、CXCR4蛋白表达均明显增高(P<0.05),提示氯化钴可上调SDF-1、CXCR4的表达;2 d后,实验组的脑细胞凋亡率明显低于模型组[(9.31±0.79)%vs(18.99±1.13)%,P<0.05];实验组脑组织梗死灶面积也明显低于模型组(P<0.05)。结论氯化钴可上调SDF-1、CXCR4蛋白表达,减少脑细胞凋亡率,缩小脑细胞缺血坏死灶面积,对缺血脑组织具有保护作用。Objective To observe the effect of cobalt chloride precondi- tioning on the expression of stromal cell derived factor （ SDF - 1 ） and CXC family chemokine receptor 4 （CXCR4） protein in rats with cerebral ischemia. Methods The 45 SD rats were randomly divided into sham group with 15 rats, model group with 15 rats, and test group with 15 rats. For model group and test group, cerebral ischemia model was established with suture emboli method. The rats in test group before modeling 2 d, 1 d were given intraperitoneal injection of cobalt chloride （30 mg. kg- 1 ）. Western blotting was used to detect the expression of SDF - 1, CXCR4 protein. Apoptosis rate of rat brain cells in each group was tested by Terminal deoxynueleotidyl transferase- mediated dUTP- biotin nick end labeling assay （TUNEL）. And after 2 days, calculate brain tissue necrosis area of the rats in model group and test group by staining with triphenyl tetrazolium chloride （TTC）. Results Compared with sham group （ SDF - 1, CXCR4 protein is very low）, the expression of SDF - 1, CXCR4 proteins in the test group and model group increased at each time point （P 〈 0. 05 ）. That was suggested that the expression of CXCR4 and SDF - 1 could be up - regulated by cobalt chloride. At time of 2 d after model established, brain cell apoptosis rate in test group was significantly lower than that in model group [ （9.31 ± 0. 79 ） % vs （ 18.99 ± 1.13 ） %, P 〈 0. 05 ]. The brain infarct area in test group was significantly lower than that in model group （P 〈 0. 05 ）. Conclusion Chromium chloride can increase the expression of SDF - 1, CXCR4 proteins, reduce the brain cell apoptosis rate, and narrow the area of necrosis of cerebral ischemia, which has a protec- tive effect for the ischemic brain tissue.

关 键 词：氯化钴 脑缺血 基质细胞衍生因子-1 趋化因子受体 预处理 

分 类 号：P07[天文地球]