PAK1蛋白和BRAFV030E突变蛋白在甲状腺乳头状癌中的表达及其临床意义  

Expression and clinical significance of PAK1 and BRAF V600E mutation in papillary thyroid carcinoma

作　　者：陈强 谢庆吉 岑宏[2] 汤聪[2] 

机构地区：[1]萧山区第一人民医院普外科,杭州311200 [2]中山大学附属第五医院甲乳外科

出　　处：《中华普通外科杂志》2016年第6期486-489,共4页Chinese Journal of General Surgery

摘　　要：目的探讨甲状腺乳头状癌（papillary thyroid carcinoma，PTC）中P21蛋白激活激酶1（P-21 activated kinase1，PAK1）和V．RAF小鼠肉瘤病毒癌基因同源B（v—raf murine sarcoma viral oncogene homolog B，BRAF）V600E突变蛋白的表达与临床病理参数之间的关系。方法免疫组化法检测55例PTC、25例良性甲状腺组织BRAFV600E突变蛋白、PAK1蛋白表达情况，分析二者与临床病理参数的关系。结果BRAF V600E突变蛋白、PAK1蛋白在PTC组织中的表达与良性甲状腺组织比较差异有统计学意义（分别为X^2=12．121，9．950，均P〈0．01）。BRAFV600E突变蛋白和PAK1蛋白的表达与FFC腺体外浸润、临床分期、淋巴结转移有关，差异有统计学意义（P〈0．05）。PAK1蛋白在BRAFV600E阴性的PTC中的表达高于其在BRAFV600E阳性的PTC中的表达,PAKI蛋白和BRAFV600E突变蛋白在甲状腺乳头状癌组织中的表达呈负相关（r=-0．284，P〈0．05）。结论BRAFV600E突变蛋白阳性表达、PAK1蛋白高表达提示PTC预后不良。Objective To investigate the expression of P-21 activated kinase 1 （PAK1） and v-raf murine sarcoma viral oneogcne homolog B （BRAF） V600E mutation in papillary thyroid carcinoma （PTC） and their relationship with clinicopathological characteristics. Methods Immunohistochemistry was used to detect the expression of BRAF V600E mutation and P-21 activated kinase 1 （ PAK1 ） in 55 PTC tissues and 25 benign thyroid tissues. The correlation between their expression and clinicopathological characteristics were analyzed. Results BRAF V6OOE mutation and PAK1 expressed differently between PTC and benign thyroid （ respectively, X^2 = 12. 121, 9. 950, all P 〈 0.01 ）. The expression of PAK1 and BRAF V600E mutation was positively and significantly correlated with tumor invasion, grades and lymph node metastasis （ P 〈 0. 05 ） , but it had no correlation with age, gender, tumor size, multiple tumor loci and bilateral tumor loci in PTC （P 〉 0. 05 ）. Expression of PAK1 in BRAF V600E negative PTC was higher than that in BRAF V600E positive PTC, and expression of PAK1 and BRAF V600E mutation in PTC were negatively correlated （ r = - 0. 284, P 〈 0. 05）. Conclusion Overexpression of either BRAF V600E mutation or PAK1 predicts poor prognosis of PTC patients.

关 键 词：甲状腺肿瘤 癌 乳头状 

分 类 号：R736.1[医药卫生—肿瘤]