EZH2在胃癌组织中的表达及对胃癌细胞增殖的影响  

Expression of PcG protein EZH2 in gastric cancer tissues and its effect on the proliferation of gastric cancer cells

作　　者：张军华[1] 崔勇[1] 桑梅香[2] 林晓萌[1] 李中[1] 张刚[1] 

机构地区：[1]河北大学附属医院乳腺外科,保定071000 [2]河北医科大学第四医院科研中心,石家庄050011

出　　处：《临床与实验病理学杂志》2016年第6期611-614,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：河北省科技支撑计划(14277732D)

摘　　要：目的探讨EZH2表达与胃癌临床病理特征、预后的关系,及其对胃癌细胞增殖的影响。方法采用免疫组化SP法检测EZH2在65例胃癌组织及20例正常胃黏膜组织中的表达,分析其表达与胃癌临床病理特征的相关性,应用Kaplan-Meier法及Log-rank检验分析EZH2表达与胃癌患者生存率的关系。采用Western blot法检测胃癌细胞株SGC7901、BGC823中EZH2的表达。EZH2 Sh RNA转染胃癌细胞株后,采用MTT法检测细胞的增殖率。结果胃癌组织中EZH2蛋白阳性率为63.07%,正常胃黏膜组织中EZH2蛋白阳性率为10%;胃癌组织中EZH2蛋白表达明显高于正常胃黏膜组织(P<0.000 1)。胃癌组织中EZH2蛋白表达与肿瘤直径(P<0.000 1)、肿瘤浸润深度(P<0.000 1)、淋巴结转移(P=0.001)及分期相关(P=0.001)。EZH2阳性者5年总生存率明显低于阴性者(P=0.001)。胃癌细胞株SGC7901和BGC823中EZH2蛋白高表达,Sh RNA介导的EZH2表达沉默可明显抑制胃癌细胞的增殖。结论 EZH2高表达是胃癌患者预后不良的重要因子,EZH2可明显促进胃癌细胞的增殖。Purpose To investigate the expression of EZH2 in human gastric cancer tissues and their correlation with the clinicopatho- logical parameters and prognosis of gastric cancer patients, and to explore its effect on the proliferation of gastric cancer ceils. Methods The immunohistochemical staining was used to examine the expression of EZH2 proteins in 65 cases of gastric cancer tissues and 20 cases of normal gastric mucosa tissues. The correlation between EZH2 expression and the clinicopathological parameters and prognosis was analyzed. Overall survival of patients was estimated by the Kaplan-Meier method, and differences between groups were compared by the Log-rank test. Western blot was used to detect the expression of EZI-I2 in gastric cancer SGC7901 and BGC823 cells. MTF assay was used to detect the cell proliferation of gastric cancer cells. Results The expression rate of EZH2 in gastric cancer tissues was 63.07%, which was significantly increased as compared with the normal gastric mucosa tissues （ 10% ） （P =0. 000 1 ）. The expression of EZH2 was positively correlated with the tumor diameter （ P = 0. 000 1 ）, tumor infiltration （ P = 0. 000 1 ）, lymph node metastasis （P =0. 001 ） and clinical stage （P =0. 001 ）. The 5-year overall survival of gastric cancer patients with EZH2 expression was significantly lower than that of patients without EZI-I2 expression. EZH2 was highly expressed in the gastric cancer SGC7901 and BGC823 cells. ShRNA-mediated EZH2 down-regulation could significantly inhibit the proliferation of gastric cancer cells. Conclusion EZH2 is highly expressed in gastric cancer tissues, and is a poor prognostic factor of gastric cancer patients. EZH2 can promote the prolifera- tion of gastric cancer cells.

关 键 词：胃肿瘤 EZH2 增殖 预后 

分 类 号：R735.2[医药卫生—肿瘤] R73-3[医药卫生—临床医学]