叶酸-壳聚糖-碳纳米管-顺铂复合物对卵巢癌细胞的体外抑瘤效应  

Antitumor effect of cisplatin-carbon nanotube-chitosan-folic acid composites on ovarian cancer in vitro

作　　者：田佳[1] 刘思思[1] 黎雁英[2] 叶元[3] 金美华[1] 周英琼[1] 

机构地区：[1]桂林医学院附属医院病理科,桂林541001 [2]桂林医学院附属医院消化内科,桂林541001 [3]桂林医学院附属医院妇产科,桂林541001

出　　处：《临床与实验病理学杂志》2016年第6期669-673,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：桂林市人口健康安全技术开发与应用研究项目(20140120-1-11)

摘　　要：目的探讨叶酸-壳聚糖-碳纳米管-顺铂(cisplatin-carbon nanotube-chitosan-folic acid,DDP-SWCNT-CHI-FA)复合物对人卵巢癌细胞的影响。方法制备DDP-SWCNT-CHI-FA复合物,测定复合物中顺铂的药载浓度;采用MTT法检测药物对卵巢癌细胞的作用;应用Western blot法检测SKOV3/DDP细胞中铜转运蛋白-1(human copper transporter-1,h CTR-1)的表达。结果 SWCNT-CHI-FA和FA-CHI携载顺铂的载药率分别为125.7%和46.08%;顺铂和DDP-SWCNT-CHI-FA对卵巢癌细胞株SKOV3的IC50分别为(8.27±0.39)μg/μl和(5.18±0.83)μg/μl,对其耐药株SKOV3/DDP的IC50分别为(37.43±1.24)μg/μl和(28.23±1.17)μg/μl;SKOV3/DDP细胞中DDP-SWCNT-CHI-FA组的h CTR-1蛋白表达水平较顺铂组低。结论制备的DDP-SWCNT-CHI-FA复合物具有高载药率;可以增强顺铂对卵巢癌顺铂耐药细胞的抑制作用。Purpose To investigate the effects of cisplatin-carbon nanotube-chitosan-folic acid （DDP-SWCNT-CHI-FA） composites on human SKOV3 ovarian cancer cells in vitro. Methods DDP-SWCNT-CHI-FA composites were prepared, The concentration of eisplatin in the DDP-SWCNT-CHI-FA composites were analyzed. The drug effects on SKOV3 cells and SKOV3/DDP cells in vitro were examined by MTT. The human copper transporter-1 （ hCTR-1 ） protein expression in the SKOVJDDP cells were detected by Western blot. Results SWCNT-CHI-FA and FA-CHI carrying eisplatin drug-loading rate were 125.7% and 46.08%. Cisplatin and DDP-SWCNT- CHI-FA ICso of ovarian cancer cell lines SKOV3 were （8.27 ±0. 39）μg/μl and （5. 18 ±0. 83） μg/μl, the IC5o of SKOV3/DDP resistant strains were （37.43 ± 1.24） μg/μl and （28.23 ±1.17 ） μg/μl. The expression level of the hCTR-1 protein in DDP-SWCNT- CHI-FA group was lower than eisplatin group in the SKOV3/DDP cells. Conclusion The DDP-SWCNT-CHI-FA composites have a high cisplatin-load rate and can enhance the inhibitory effect of cisplatin on SKOV3/DDP ovarian cancer cells. The mechanism may be related to down regulate hCTR-1 protein expression.

关 键 词：卵巢肿瘤 叶酸-壳聚糖-碳纳米管-顺铂复合物 铜转运蛋白-1 耐药 

分 类 号：R737.31[医药卫生—肿瘤] R73-3[医药卫生—临床医学]